Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process

Jeong Chan Joo, Young Hoon Oh, Ju Hyun Yu, Sung Min Hyun, Tae Uk Khang, Kyoung Hee Kang, Bong Keun Song, Kyungmoon Park, Min-Kyu Oh, Sang Yup Lee, Si Jae Park

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

This study examined nine expired industrial Corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-AVA. C. glutamicum KCTC 1857 exhibiting the highest lysine production was transformed with either original Pseudomonas putida davBA genes, encoding the 5-AVA biosynthesis pathway, or C. glutamicum codon-optimized davBA genes. C. glutamicum KCTC 1857 expressing the original genes had superior cell viability and 5-AVA production capability compared to the other strain. This strain produced 39.93. g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose. Indeed, Miscanthus hydrolysate solution prepared from a novel process, comprising pretreatment, hydrolysis, purification, and concentration, was used as feedstock for 5-AVA production. A total of 12.51. g/L 5-AVA was produced from the Miscanthus hydrolysate; this value is 34.7% higher than that obtained from glucose in batch fermentation.

Original languageEnglish
JournalBioresource Technology
DOIs
Publication statusAccepted/In press - 2017 Mar 29

Keywords

  • 5-Aminovaleric acid
  • Corynebacterium glutamicum
  • Metabolic engineering
  • Miscanthus
  • Pretreatment

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

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