Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process

Jeong Chan Joo, Young Hoon Oh, Ju Hyun Yu, Sung Min Hyun, Tae Uk Khang, Kyoung Hee Kang, Bong Keun Song, Kyungmoon Park, Min-Kyu Oh, Sang Yup Lee, Si Jae Park

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study examined nine expired industrial Corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-AVA. C. glutamicum KCTC 1857 exhibiting the highest lysine production was transformed with either original Pseudomonas putida davBA genes, encoding the 5-AVA biosynthesis pathway, or C. glutamicum codon-optimized davBA genes. C. glutamicum KCTC 1857 expressing the original genes had superior cell viability and 5-AVA production capability compared to the other strain. This strain produced 39.93. g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose. Indeed, Miscanthus hydrolysate solution prepared from a novel process, comprising pretreatment, hydrolysis, purification, and concentration, was used as feedstock for 5-AVA production. A total of 12.51. g/L 5-AVA was produced from the Miscanthus hydrolysate; this value is 34.7% higher than that obtained from glucose in batch fermentation.

Original languageEnglish
JournalBioresource Technology
DOIs
Publication statusAccepted/In press - 2017 Mar 29

Fingerprint

hydrolysis
Hydrolysis
fermentation
Acids
gene
acid
glucose
Fermentation
Lysine
Glucose
Genes
Gene encoding
purification
Biosynthesis
viability
Feedstocks
Purification
Cells
5-aminovaleric acid
biosynthesis

Keywords

  • 5-Aminovaleric acid
  • Corynebacterium glutamicum
  • Metabolic engineering
  • Miscanthus
  • Pretreatment

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process. / Joo, Jeong Chan; Oh, Young Hoon; Yu, Ju Hyun; Hyun, Sung Min; Khang, Tae Uk; Kang, Kyoung Hee; Song, Bong Keun; Park, Kyungmoon; Oh, Min-Kyu; Lee, Sang Yup; Park, Si Jae.

In: Bioresource Technology, 29.03.2017.

Research output: Contribution to journalArticle

Joo, Jeong Chan ; Oh, Young Hoon ; Yu, Ju Hyun ; Hyun, Sung Min ; Khang, Tae Uk ; Kang, Kyoung Hee ; Song, Bong Keun ; Park, Kyungmoon ; Oh, Min-Kyu ; Lee, Sang Yup ; Park, Si Jae. / Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process. In: Bioresource Technology. 2017.
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