Production of a novel cold-active lipase from Pichia lynferdii Y-7723

Hak Ryul Kim, I. N.Hwan Kim, Chino T. Hou, Kwang I.L. Kwon, Beom Soo Shin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Lipase (triacylglycerol acylhydrolases, E.C. 3.1.1.3) is one of the most important enzymes applied to a broad range of industrial application fields. Especially, lipases with abnormal functionality such as thermostability and alkaline, acidic, and cold activities gain special attention because of their applicability in the restricted reaction conditions. In this study, 16 yeast strains prescreened for lipase induction were investigated for their actual lipase production, and we found a novel coldactive lipase produced from Pichia lynferdii Y-7723. The activity of lipase Y-7723 was retained by 74 and 70% at 20 and 10 °C, respectively, as compared to the maximum value at 35 °C. On the basis of an optimization study, the optimal lipase productivity was obtained at 96 h of incubation with 3% oil substrate in a medium composed of sucrose as a carbon source at pH 7.0. Among carbon sources tested, sucrose showed almost twice as high of a lipase production (184%) as the control, while the cell growth was similar (105%). Yeast extract and ammonium salts were effective as individual nitrogen sources for lipase production. This study demonstrated that the cold activity of lipase Y-7723 at 10 °C was highest among the cold-active lipases reported so far.

Original languageEnglish
Pages (from-to)1322-1326
Number of pages5
JournalJournal of agricultural and food chemistry
Volume58
Issue number2
DOIs
Publication statusPublished - 2010 Jan 27

Keywords

  • Cold-active
  • Lipase
  • Optimization
  • Pichia lynferdii
  • Yeast

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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