Production of biofunctional recombinant human interleukin 1 receptor antagonist (rhIL1RN) from transgenic quail egg white

Se Chang Kwon, Jin Won Choi, Hyun Jun Jang, Sang Su Shin, Seul Ki Lee, Tae Sub Park, In Young Choi, Gwan Sun Lee, Gwonhwa Song, Jae Yong Han

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29 Citations (Scopus)

Abstract

Oviduct-specific expression of heterologous recombinant proteins in transgenic birds is a promising technology for the large-scale production of therapeutic proteins in eggs. We describe the production of recombinant human interleukin 1 receptor antagonist (rhIL1RN) in the eggs of transgenic quails. To drive tissue-specific expression of rhIL1RN, a 1.35-kb fragment of the chicken ovalbumin promoter, which contains both the steroid-dependent regulatory element and the negative regulatory element, was used. A transgenic quail was generated by microinjection of a concentrated stock of lentivirus into stage X blastodermal cells. A single copy of the transgene was integrated into the seventh intron of the gene for conserved oligomeric golgi complex protein 5 (COG5) on chromosome 1. As expected, rhIL1RN expression was restricted to oviductal tissue, and the amount of protein deposited in the eggs of homozygous transgenic quails ranged from 88.7 to 233.8 ng/ml. Transgene expression was conserved from the G1 generation to the G4 generation, and there was no evidence of transgene silencing. In a bioassay using the EL4.NOB-1/CTLL-2 coculture system, no significant difference was observed between the egg-produced rhIL1RN and a commercially available rhIL1RN (anakinra).

Original languageEnglish
Pages (from-to)1057-1064
Number of pages8
JournalBiology of reproduction
Volume82
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1
Externally publishedYes

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Keywords

  • Chicken ovalbumin promoter
  • Embryo
  • Gene regulation
  • Lentiviral vector
  • Oviduct
  • Transgenic quail
  • rhIL1RN

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Kwon, S. C., Choi, J. W., Jang, H. J., Shin, S. S., Lee, S. K., Park, T. S., Choi, I. Y., Lee, G. S., Song, G., & Han, J. Y. (2010). Production of biofunctional recombinant human interleukin 1 receptor antagonist (rhIL1RN) from transgenic quail egg white. Biology of reproduction, 82(6), 1057-1064. https://doi.org/10.1095/biolreprod.109.081687