Production of cellulases and β-glucosidase in Trichoderma reesei mutated by proton beam irradiation

You Ree Jung, Hyun Yong Shin, Hah Young Yoo, Youngsoon Um, Seung Wook Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To obtain mutant strains producing high levels of cellulases (FPase and CMCase) and β-glucosidase, Trichoderma reesei KCTC 6950 was mutated by proton beam irradiation. Five mutants were selected out of 1,000 mutants of T. reesei treated with proton beam irradiation, based on their ability for enzyme production on a plate screening medium. In submerged cultures containing Mandel's fermentation medium, the mutant strain T-2 (MT-2) demonstrated a 165% increase in the activity of FPase, a 146% increase in the activity of CMCase, and a 313% increase in the activity of β-glucosidase, compared with the wild type strain. Additionally, the properties of high level β-glucosidase produced by MT-2 were the same as those of the wild type strain, e. g., an optimum pH of 4.8, and an optimum temperature of 65 °C. Moreover, the protein concentrations of β-glucosidase produced by the wild type strain and MT-2 were measured by SDS-PAGE, and then β-glucosidase activities were detected by the MUG-zymogram assay.

Original languageEnglish
Pages (from-to)925-930
Number of pages6
JournalKorean Journal of Chemical Engineering
Volume29
Issue number7
DOIs
Publication statusPublished - 2012 Jul

Keywords

  • Cellulases
  • Mutation
  • Proton Beam Irradiation
  • Trichoderma reesei
  • β-Glucosidase

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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