Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes

Eugene Jeon, Jeong E. Hyeon, Dong Jin Suh, Young Woong Suh, Seung Wook Kim, Kwang Ho Song, Sung Ok Han

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.

Original languageEnglish
Pages (from-to)369-373
Number of pages5
JournalMolecules and Cells
Volume28
Issue number4
DOIs
Publication statusPublished - 2009 Oct 13

Fingerprint

Saccharomycopsis
Clostridium thermocellum
Glucosidases
Cellulase
Saccharomyces cerevisiae
Ethanol
Yeasts
Mating Factor
Cellulose
Fermentation
Enzymes
Genes
Glucose
Glucans
Protein Sorting Signals
Acids

Keywords

  • Beta-glucosidase
  • Cellulose degradation
  • Clostridium thermocellum
  • Endoglucanase
  • Ethanol production
  • Extracellular expression
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes. / Jeon, Eugene; Hyeon, Jeong E.; Suh, Dong Jin; Suh, Young Woong; Kim, Seung Wook; Song, Kwang Ho; Han, Sung Ok.

In: Molecules and Cells, Vol. 28, No. 4, 13.10.2009, p. 369-373.

Research output: Contribution to journalArticle

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AU - Suh, Dong Jin

AU - Suh, Young Woong

AU - Kim, Seung Wook

AU - Song, Kwang Ho

AU - Han, Sung Ok

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