Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes

Eugene Jeon, Jeong Eun Hyeon, Dong Jin Suh, Young Woong Suh, Seoung Wook Kim, Kwang Ho Song, Sung Ok Han

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.

Original languageEnglish
Pages (from-to)369-373
Number of pages5
JournalMolecules and cells
Volume28
Issue number4
DOIs
Publication statusPublished - 2009

Keywords

  • Beta-glucosidase
  • Cellulose degradation
  • Clostridium thermocellum
  • Endoglucanase
  • Ethanol production
  • Extracellular expression
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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