Abstract
Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.
| Original language | English |
|---|---|
| Pages (from-to) | 369-373 |
| Number of pages | 5 |
| Journal | Molecules and Cells |
| Volume | 28 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2009 Oct 13 |
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Keywords
- Beta-glucosidase
- Cellulose degradation
- Clostridium thermocellum
- Endoglucanase
- Ethanol production
- Extracellular expression
- Saccharomyces cerevisiae
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes. / Jeon, Eugene; Hyeon, Jeong E.; Suh, Dong Jin; Suh, Young Woong; Kim, Seung Wook; Song, Kwang Ho; Han, Sung Ok.
In: Molecules and Cells, Vol. 28, No. 4, 13.10.2009, p. 369-373.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes
AU - Jeon, Eugene
AU - Hyeon, Jeong E.
AU - Suh, Dong Jin
AU - Suh, Young Woong
AU - Kim, Seung Wook
AU - Song, Kwang Ho
AU - Han, Sung Ok
PY - 2009/10/13
Y1 - 2009/10/13
N2 - Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.
AB - Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.
KW - Beta-glucosidase
KW - Cellulose degradation
KW - Clostridium thermocellum
KW - Endoglucanase
KW - Ethanol production
KW - Extracellular expression
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=72449144777&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=72449144777&partnerID=8YFLogxK
U2 - 10.1007/s10059-009-0131-y
DO - 10.1007/s10059-009-0131-y
M3 - Article
VL - 28
SP - 369
EP - 373
JO - Molecules and Cells
JF - Molecules and Cells
SN - 1016-8478
IS - 4
ER -