Abstract
Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.
| Original language | English |
|---|---|
| Pages (from-to) | 369-373 |
| Number of pages | 5 |
| Journal | Molecules and cells |
| Volume | 28 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2009 |
Keywords
- Beta-glucosidase
- Cellulose degradation
- Clostridium thermocellum
- Endoglucanase
- Ethanol production
- Extracellular expression
- Saccharomyces cerevisiae
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
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Jeon, E., Hyeon, J. E., Suh, D. J., Suh, Y. W., Kim, S. W., Song, K. H., & Han, S. O. (2009). Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes. Molecules and cells, 28(4), 369-373. https://doi.org/10.1007/s10059-009-0131-y