Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes

Eugene Jeon; Jeong Eun Hyeon; Dong Jin Suh; Young Woong Suh; Seoung Wook Kim; Kwang Ho Song; Sung Ok Han

doi:10.1007/s10059-009-0131-y

Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes

Eugene Jeon, Jeong Eun Hyeon, Dong Jin Suh, Young Woong Suh, Seoung Wook Kim, Kwang Ho Song, Sung Ok Han

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.

Original language	English
Pages (from-to)	369-373
Number of pages	5
Journal	Molecules and cells
Volume	28
Issue number	4
DOIs	https://doi.org/10.1007/s10059-009-0131-y
Publication status	Published - 2009

Keywords

Beta-glucosidase
Cellulose degradation
Clostridium thermocellum
Endoglucanase
Ethanol production
Extracellular expression
Saccharomyces cerevisiae

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes. / Jeon, Eugene; Hyeon, Jeong Eun; Suh, Dong Jin; Suh, Young Woong; Kim, Seoung Wook ; Song, Kwang Ho ; Han, Sung Ok.

In: Molecules and cells, Vol. 28, No. 4, 2009, p. 369-373.

Research output: Contribution to journal › Article › peer-review

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title = "Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes",

abstract = "Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.",

keywords = "Beta-glucosidase, Cellulose degradation, Clostridium thermocellum, Endoglucanase, Ethanol production, Extracellular expression, Saccharomyces cerevisiae",

author = "Eugene Jeon and Hyeon, {Jeong Eun} and Suh, {Dong Jin} and Suh, {Young Woong} and Kim, {Seoung Wook} and Song, {Kwang Ho} and Han, {Sung Ok}",

note = "Funding Information: We thank Roy H. Doi (University of California, Davis) for critical reading of the manuscript. This work was supported in part by the Korea Science and Tngineering Foundation grant funded by the Korea Government (Ministry of Science and Technology) (No. R01-2008-000-20438-0) and National Research Foundation of Korea Grant funded by the Korea Government (2009-0073856).",

year = "2009",

doi = "10.1007/s10059-009-0131-y",

language = "English",

volume = "28",

pages = "369--373",

journal = "Molecules and Cells",

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T1 - Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes

AU - Jeon, Eugene

AU - Hyeon, Jeong Eun

AU - Suh, Dong Jin

AU - Suh, Young Woong

AU - Kim, Seoung Wook

AU - Song, Kwang Ho

AU - Han, Sung Ok

N1 - Funding Information: We thank Roy H. Doi (University of California, Davis) for critical reading of the manuscript. This work was supported in part by the Korea Science and Tngineering Foundation grant funded by the Korea Government (Ministry of Science and Technology) (No. R01-2008-000-20438-0) and National Research Foundation of Korea Grant funded by the Korea Government (2009-0073856).

PY - 2009

Y1 - 2009

N2 - Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.

AB - Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.

KW - Beta-glucosidase

KW - Cellulose degradation

KW - Clostridium thermocellum

KW - Endoglucanase

KW - Ethanol production

KW - Extracellular expression

KW - Saccharomyces cerevisiae

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U2 - 10.1007/s10059-009-0131-y

DO - 10.1007/s10059-009-0131-y

M3 - Article

C2 - 19812901

AN - SCOPUS:72449144777

VL - 28

SP - 369

EP - 373

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 4

ER -

Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes

Abstract

Keywords

ASJC Scopus subject areas

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