Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35

Hyun Yong Shin; Jin Young Lee; Han Suk Choi; Ja Hyun Lee; Seung Wook Kim

doi:10.1007/s12275-011-1155-5

Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35

Hyun Yong Shin, Jin Young Lee, Han Suk Choi, Ja Hyun Lee, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

In this study, cephalosporin C production by Acremonium chrysogenum M35 cultured with crude glycerol instead of rice oil and methionine was investigated. The addition of crude glycerol increased cephalosporin C production by 6-fold in shake-flask culture, and also the amount of cysteine. In fed-batch culture without methionine, crude glycerol resulted only in overall improvement in cephalosporin C production (about 700%). In addition, A. chrysogenum M35 became highly differentiated in fed-batch culture with crude glycerol, compared with the differentiation in batch culture. The results presented here suggest that crude glycerol can replace methionine and plant oil as cysteine and carbon sources during cephalosporin C production by A. chrysogenum M35.

Original language	English
Pages (from-to)	753-758
Number of pages	6
Journal	Journal of Microbiology
Volume	49
Issue number	5
DOIs	https://doi.org/10.1007/s12275-011-1155-5
Publication status	Published - 2011 Oct

Keywords

Acremonium chrysogenum M35
cephalosporin C
crude glycerol
cystathionine-γ-lyase activity
cysteine
methionine

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

Access to Document

10.1007/s12275-011-1155-5

Fingerprint Dive into the research topics of 'Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35'. Together they form a unique fingerprint.

Cite this

@article{246e0279cbbf4363a05cb6d13554545b,

title = "Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35",

abstract = "In this study, cephalosporin C production by Acremonium chrysogenum M35 cultured with crude glycerol instead of rice oil and methionine was investigated. The addition of crude glycerol increased cephalosporin C production by 6-fold in shake-flask culture, and also the amount of cysteine. In fed-batch culture without methionine, crude glycerol resulted only in overall improvement in cephalosporin C production (about 700%). In addition, A. chrysogenum M35 became highly differentiated in fed-batch culture with crude glycerol, compared with the differentiation in batch culture. The results presented here suggest that crude glycerol can replace methionine and plant oil as cysteine and carbon sources during cephalosporin C production by A. chrysogenum M35.",

keywords = "Acremonium chrysogenum M35, cephalosporin C, crude glycerol, cystathionine-γ-lyase activity, cysteine, methionine",

author = "Shin, {Hyun Yong} and Lee, {Jin Young} and Choi, {Han Suk} and Lee, {Ja Hyun} and Kim, {Seung Wook}",

note = "Funding Information: This work was supported by the Advenced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science and Technology (ABC-2010-0029799) and the core Environmental Technology Development project for Next Generation funded by the Ministry of Environment, Republic of Korea (No.032-091-019).",

year = "2011",

month = oct,

doi = "10.1007/s12275-011-1155-5",

language = "English",

volume = "49",

pages = "753--758",

journal = "Journal of Microbiology",

issn = "1225-8873",

publisher = "Microbiological Society of Korea",

number = "5",

}

TY - JOUR

T1 - Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35

AU - Shin, Hyun Yong

AU - Lee, Jin Young

AU - Choi, Han Suk

AU - Lee, Ja Hyun

AU - Kim, Seung Wook

N1 - Funding Information: This work was supported by the Advenced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science and Technology (ABC-2010-0029799) and the core Environmental Technology Development project for Next Generation funded by the Ministry of Environment, Republic of Korea (No.032-091-019).

PY - 2011/10

Y1 - 2011/10

N2 - In this study, cephalosporin C production by Acremonium chrysogenum M35 cultured with crude glycerol instead of rice oil and methionine was investigated. The addition of crude glycerol increased cephalosporin C production by 6-fold in shake-flask culture, and also the amount of cysteine. In fed-batch culture without methionine, crude glycerol resulted only in overall improvement in cephalosporin C production (about 700%). In addition, A. chrysogenum M35 became highly differentiated in fed-batch culture with crude glycerol, compared with the differentiation in batch culture. The results presented here suggest that crude glycerol can replace methionine and plant oil as cysteine and carbon sources during cephalosporin C production by A. chrysogenum M35.

AB - In this study, cephalosporin C production by Acremonium chrysogenum M35 cultured with crude glycerol instead of rice oil and methionine was investigated. The addition of crude glycerol increased cephalosporin C production by 6-fold in shake-flask culture, and also the amount of cysteine. In fed-batch culture without methionine, crude glycerol resulted only in overall improvement in cephalosporin C production (about 700%). In addition, A. chrysogenum M35 became highly differentiated in fed-batch culture with crude glycerol, compared with the differentiation in batch culture. The results presented here suggest that crude glycerol can replace methionine and plant oil as cysteine and carbon sources during cephalosporin C production by A. chrysogenum M35.

KW - Acremonium chrysogenum M35

KW - cephalosporin C

KW - crude glycerol

KW - cystathionine-γ-lyase activity

KW - cysteine

KW - methionine

UR - http://www.scopus.com/inward/record.url?scp=80955131435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80955131435&partnerID=8YFLogxK

U2 - 10.1007/s12275-011-1155-5

DO - 10.1007/s12275-011-1155-5

M3 - Article

C2 - 22068491

AN - SCOPUS:80955131435

VL - 49

SP - 753

EP - 758

JO - Journal of Microbiology

JF - Journal of Microbiology

SN - 1225-8873

IS - 5

ER -

Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this