Abstract
In this study, cephalosporin C production by Acremonium chrysogenum M35 cultured with crude glycerol instead of rice oil and methionine was investigated. The addition of crude glycerol increased cephalosporin C production by 6-fold in shake-flask culture, and also the amount of cysteine. In fed-batch culture without methionine, crude glycerol resulted only in overall improvement in cephalosporin C production (about 700%). In addition, A. chrysogenum M35 became highly differentiated in fed-batch culture with crude glycerol, compared with the differentiation in batch culture. The results presented here suggest that crude glycerol can replace methionine and plant oil as cysteine and carbon sources during cephalosporin C production by A. chrysogenum M35.
| Original language | English |
|---|---|
| Pages (from-to) | 753-758 |
| Number of pages | 6 |
| Journal | Journal of Microbiology |
| Volume | 49 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2011 Oct 1 |
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Keywords
- Acremonium chrysogenum M35
- cephalosporin C
- crude glycerol
- cystathionine-γ-lyase activity
- cysteine
- methionine
ASJC Scopus subject areas
- Microbiology
- Applied Microbiology and Biotechnology
Cite this
Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35. / Shin, Hyun Yong; Lee, Jin Young; Choi, Han Suk; Lee, Ja Hyun; Kim, Seung Wook.
In: Journal of Microbiology, Vol. 49, No. 5, 01.10.2011, p. 753-758.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35
AU - Shin, Hyun Yong
AU - Lee, Jin Young
AU - Choi, Han Suk
AU - Lee, Ja Hyun
AU - Kim, Seung Wook
PY - 2011/10/1
Y1 - 2011/10/1
N2 - In this study, cephalosporin C production by Acremonium chrysogenum M35 cultured with crude glycerol instead of rice oil and methionine was investigated. The addition of crude glycerol increased cephalosporin C production by 6-fold in shake-flask culture, and also the amount of cysteine. In fed-batch culture without methionine, crude glycerol resulted only in overall improvement in cephalosporin C production (about 700%). In addition, A. chrysogenum M35 became highly differentiated in fed-batch culture with crude glycerol, compared with the differentiation in batch culture. The results presented here suggest that crude glycerol can replace methionine and plant oil as cysteine and carbon sources during cephalosporin C production by A. chrysogenum M35.
AB - In this study, cephalosporin C production by Acremonium chrysogenum M35 cultured with crude glycerol instead of rice oil and methionine was investigated. The addition of crude glycerol increased cephalosporin C production by 6-fold in shake-flask culture, and also the amount of cysteine. In fed-batch culture without methionine, crude glycerol resulted only in overall improvement in cephalosporin C production (about 700%). In addition, A. chrysogenum M35 became highly differentiated in fed-batch culture with crude glycerol, compared with the differentiation in batch culture. The results presented here suggest that crude glycerol can replace methionine and plant oil as cysteine and carbon sources during cephalosporin C production by A. chrysogenum M35.
KW - Acremonium chrysogenum M35
KW - cephalosporin C
KW - crude glycerol
KW - cystathionine-γ-lyase activity
KW - cysteine
KW - methionine
UR - http://www.scopus.com/inward/record.url?scp=80955131435&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80955131435&partnerID=8YFLogxK
U2 - 10.1007/s12275-011-1155-5
DO - 10.1007/s12275-011-1155-5
M3 - Article
VL - 49
SP - 753
EP - 758
JO - Journal of Microbiology
JF - Journal of Microbiology
SN - 1225-8873
IS - 5
ER -