Production of functional agarolytic nano-complex for the synergistic hydrolysis of marine biomass and its potential application in carbohydrate-binding module-utilizing one-step purification

Jeong Eun Hyeon, Dae Hee Kang, Young In Kim, Sang Duck Jeon, Seung Kyou You, Kyung Yeon Kim, Seung Wook Kim, Sung Ok Han

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Marine algal polysaccharides are promising alternative resources to terrestrial biomass. Agarolytic enzymes degrade agarose to various kinds of oligosaccharides. In this study, we expressed miniCbpA, a recombinant scaffolding protein from Clostridium cellulovorans, in Escherichia coli. To assemble the agarolytic complex via cohesin-dockerin interaction, we constructed a chimeric agarase cAgaB from Zobellia galactanivorans containing the catalytic domain of AgaB fused with a dockerin domain from C. cellulovorans EngB. The assembly of functional agarolytic complexes increased the activity against the agar substrate approximately 1.4-fold compared with that for the corresponding enzymes alone. The carbohydrate-binding module (CBM) of miniCbpA was used as a tag for CBM-utilizing one-step purification using cellulose as a support. This is the first report on the formation of agarolytic complexes using the cohesin-dockerin interaction system. The assembly of agar-degrading complexes will lead to the commercial production of useful products from agar biomass at low costs.

Original languageEnglish
Pages (from-to)877-881
Number of pages5
JournalProcess Biochemistry
Volume47
Issue number5
DOIs
Publication statusPublished - 2012 May 1

Keywords

  • β-Agarase
  • Agar
  • Agarolytic complex
  • Carbohydrate-binding module
  • Scaffolding protein
  • Zobellia galactanivorans

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

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