Production of functional agarolytic nano-complex for the synergistic hydrolysis of marine biomass and its potential application in carbohydrate-binding module-utilizing one-step purification

Jeong Eun Hyeon, Dae Hee Kang, Young In Kim, Sang Duck Jeon, Seung Kyou You, Kyung Yeon Kim, Seung Wook Kim, Sung Ok Han

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Marine algal polysaccharides are promising alternative resources to terrestrial biomass. Agarolytic enzymes degrade agarose to various kinds of oligosaccharides. In this study, we expressed miniCbpA, a recombinant scaffolding protein from Clostridium cellulovorans, in Escherichia coli. To assemble the agarolytic complex via cohesin-dockerin interaction, we constructed a chimeric agarase cAgaB from Zobellia galactanivorans containing the catalytic domain of AgaB fused with a dockerin domain from C. cellulovorans EngB. The assembly of functional agarolytic complexes increased the activity against the agar substrate approximately 1.4-fold compared with that for the corresponding enzymes alone. The carbohydrate-binding module (CBM) of miniCbpA was used as a tag for CBM-utilizing one-step purification using cellulose as a support. This is the first report on the formation of agarolytic complexes using the cohesin-dockerin interaction system. The assembly of agar-degrading complexes will lead to the commercial production of useful products from agar biomass at low costs.

Original languageEnglish
Pages (from-to)877-881
Number of pages5
JournalProcess Biochemistry
Volume47
Issue number5
DOIs
Publication statusPublished - 2012 May

Keywords

  • Agar
  • Agarolytic complex
  • Carbohydrate-binding module
  • Scaffolding protein
  • Zobellia galactanivorans
  • β-Agarase

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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