Production of HBsAg by growth rate control with recombinant Saccharomycescerevisiae in fed-batch

Man Bock Gu; Kyung Hwan Jung; Mahn Hoon Park; Kwang Soon Shin; Kyong Ho Kim

doi:10.1007/BF01026776

Production of HBsAg by growth rate control with recombinant Saccharomycescerevisiae in fed-batch

Man Bock Gu, Kyung Hwan Jung, Mahn Hoon Park, Kwang Soon Shin, Kyong Ho Kim

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

To maintain a constant specific growth rate for a recombinant S. cerevisiae in fed-batch, the medium feeding rate has been controlled with respect to the hourly calculated glucose uptake rate. The recombinant yeast producing HBsAg showed the exponential production trend in proportion to the exponential cell growth. Total cell yield in fed-batch was about 0.402 g cells/g glucose, and HBsAg was produced about ten times more than in batch. Decrease of growth rate by HBsAg produced was not shown.

Original language	English
Pages (from-to)	1-4
Number of pages	4
Journal	Biotechnology letters
Volume	11
Issue number	1
DOIs	https://doi.org/10.1007/BF01026776
Publication status	Published - 1989 Jan
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF01026776

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abstract = "To maintain a constant specific growth rate for a recombinant S. cerevisiae in fed-batch, the medium feeding rate has been controlled with respect to the hourly calculated glucose uptake rate. The recombinant yeast producing HBsAg showed the exponential production trend in proportion to the exponential cell growth. Total cell yield in fed-batch was about 0.402 g cells/g glucose, and HBsAg was produced about ten times more than in batch. Decrease of growth rate by HBsAg produced was not shown.",

author = "Gu, {Man Bock} and Jung, {Kyung Hwan} and Park, {Mahn Hoon} and Shin, {Kwang Soon} and Kim, {Kyong Ho}",

year = "1989",

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AU - Gu, Man Bock

AU - Jung, Kyung Hwan

AU - Park, Mahn Hoon

AU - Shin, Kwang Soon

AU - Kim, Kyong Ho

PY - 1989/1

Y1 - 1989/1

N2 - To maintain a constant specific growth rate for a recombinant S. cerevisiae in fed-batch, the medium feeding rate has been controlled with respect to the hourly calculated glucose uptake rate. The recombinant yeast producing HBsAg showed the exponential production trend in proportion to the exponential cell growth. Total cell yield in fed-batch was about 0.402 g cells/g glucose, and HBsAg was produced about ten times more than in batch. Decrease of growth rate by HBsAg produced was not shown.

AB - To maintain a constant specific growth rate for a recombinant S. cerevisiae in fed-batch, the medium feeding rate has been controlled with respect to the hourly calculated glucose uptake rate. The recombinant yeast producing HBsAg showed the exponential production trend in proportion to the exponential cell growth. Total cell yield in fed-batch was about 0.402 g cells/g glucose, and HBsAg was produced about ten times more than in batch. Decrease of growth rate by HBsAg produced was not shown.

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DO - 10.1007/BF01026776

M3 - Article

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JF - Biotechnology Letters

IS - 1

ER -

Production of HBsAg by growth rate control with recombinant Saccharomycescerevisiae in fed-batch

Abstract

ASJC Scopus subject areas

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