Production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant Corynebacterium glutamicum

Jeong Eun Hyeon, Woo Jung Jeon, Sun Young Whang, Sung Ok Han

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Although cellulosic materials of plant origin are the most abundant utilizable biomass resource, the amino acid-producing organism Corynebacterium glutamicum can not utilize these materials. Here we report the engineering of a C. glutamicum strain expressing functional minicellulosomes containing chimeric endoglucanase E bound to miniCbpA from Clostridium cellulovorans that can hydrolyze cellulosic materials. The chimeric endoglucanase E consists of the endoglucanase E catalytic backbone of Clostridium thermocellum fused with the endoglucanase B dockerin domain of C. cellulovorans. The resulting strain degraded cellulose efficiently by substrate targeting via the carbohydrate binding module. The assembly of minicellulosomes increased the activity against carboxymethyl cellulose approximately 2.8-fold compared with that for the corresponding enzymes alone. This is the first report of the formation of Clostridium minicellulosomes by C. glutamicum. The development of C. glutamicum strain that is capable of more effective cellulose hydrolysis brings about a realization of consolidated bioprocessing for the utilization of cellulosic biomass.

Original languageEnglish
Pages (from-to)371-377
Number of pages7
JournalEnzyme and Microbial Technology
Volume48
Issue number4-5
DOIs
Publication statusPublished - 2011 Apr 7

Fingerprint

Corynebacterium glutamicum
Cellulase
Clostridium
Clostridium cellulovorans
Hydrolysis
Substrates
Cellulose
Biomass
Clostridium thermocellum
Carboxymethylcellulose Sodium
Carbohydrates
Amino Acids
Enzymes

Keywords

  • Cellulase
  • Clostridium cellulovorans
  • Consolidated bioprocessing
  • Corynebacterium glutamicum
  • Minicellulosome

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant Corynebacterium glutamicum. / Hyeon, Jeong Eun; Jeon, Woo Jung; Whang, Sun Young; Han, Sung Ok.

In: Enzyme and Microbial Technology, Vol. 48, No. 4-5, 07.04.2011, p. 371-377.

Research output: Contribution to journalArticle

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