Abstract
Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl- cellulose (CMC)] into ethanol with the aid of β-glucosidase 1 produced from Saccharomycopsis fibuligera. The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulose-binding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L-1 initial CMC, approximately 3.45 g L-1 ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g-1 from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes.
| Original language | English |
|---|---|
| Pages (from-to) | 39-47 |
| Number of pages | 9 |
| Journal | FEMS Microbiology Letters |
| Volume | 310 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2010 Sep 1 |
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Keywords
- cellulose-binding domain
- Clostridium cellulovorans
- consolidated bioprocessing
- ethanol fermentation
- minicellulosome
- Saccharomyces cerevisiae
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Microbiology
Cite this
Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae. / Hyeon, Jeong E.; Yu, Kyung O.; Suh, Dong Jin; Suh, Young Woong; Lee, Sung Eun; Lee, Jinwon; Han, Sung Ok.
In: FEMS Microbiology Letters, Vol. 310, No. 1, 01.09.2010, p. 39-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae
AU - Hyeon, Jeong E.
AU - Yu, Kyung O.
AU - Suh, Dong Jin
AU - Suh, Young Woong
AU - Lee, Sung Eun
AU - Lee, Jinwon
AU - Han, Sung Ok
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl- cellulose (CMC)] into ethanol with the aid of β-glucosidase 1 produced from Saccharomycopsis fibuligera. The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulose-binding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L-1 initial CMC, approximately 3.45 g L-1 ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g-1 from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes.
AB - Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl- cellulose (CMC)] into ethanol with the aid of β-glucosidase 1 produced from Saccharomycopsis fibuligera. The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulose-binding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L-1 initial CMC, approximately 3.45 g L-1 ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g-1 from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes.
KW - cellulose-binding domain
KW - Clostridium cellulovorans
KW - consolidated bioprocessing
KW - ethanol fermentation
KW - minicellulosome
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=77955266119&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955266119&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2010.02035.x
DO - 10.1111/j.1574-6968.2010.02035.x
M3 - Article
C2 - 20637040
AN - SCOPUS:77955266119
VL - 310
SP - 39
EP - 47
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1
ER -