Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae

Jeong E. Hyeon, Kyung O. Yu, Dong Jin Suh, Young Woong Suh, Sung Eun Lee, Jinwon Lee, Sung Ok Han

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl- cellulose (CMC)] into ethanol with the aid of β-glucosidase 1 produced from Saccharomycopsis fibuligera. The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulose-binding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L-1 initial CMC, approximately 3.45 g L-1 ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g-1 from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes.

Original languageEnglish
Pages (from-to)39-47
Number of pages9
JournalFEMS Microbiology Letters
Volume310
Issue number1
DOIs
Publication statusPublished - 2010 Sep 1

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Keywords

  • cellulose-binding domain
  • Clostridium cellulovorans
  • consolidated bioprocessing
  • ethanol fermentation
  • minicellulosome
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Microbiology

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