Aryl acylamidase (EC 3. 5. 1. 13, AAA) acts on the amide bond between aryl and acyl groups. Whole cells of Escherichia coli overexpressing a novel bacterial AAA synthesized p-acetaminophenol (p-AAP) from p-aminophenol (p-AP, aryl compound) and acetate (acyl donor). Optimum conditions were pH 5.5 and 35°C with 100 mM p-AP and 600 mM sodium acetate in 100 mM sodium phosphate buffer including 1% (v/v) Triton X-100 for 60 h. 13.1 g p-AAP l -1 was produced with a conversion yield of 87%.
- Aryl acylamidase
- Whole-cell catalysis
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology