Production of p-acetaminophenol by whole-cell catalysis using Escherichia coli overexpressing bacterial aryl acylamidase

Hyeok Jin Ko; Won Gi Bang; Kyoung Heon Kim; In-Geol Choi

doi:10.1007/s10529-011-0811-5

Production of p-acetaminophenol by whole-cell catalysis using Escherichia coli overexpressing bacterial aryl acylamidase

Hyeok Jin Ko, Won Gi Bang, Kyoung Heon Kim, In-Geol Choi

Department of Biotechnology

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

Aryl acylamidase (EC 3. 5. 1. 13, AAA) acts on the amide bond between aryl and acyl groups. Whole cells of Escherichia coli overexpressing a novel bacterial AAA synthesized p-acetaminophenol (p-AAP) from p-aminophenol (p-AP, aryl compound) and acetate (acyl donor). Optimum conditions were pH 5.5 and 35°C with 100 mM p-AP and 600 mM sodium acetate in 100 mM sodium phosphate buffer including 1% (v/v) Triton X-100 for 60 h. 13.1 g p-AAP l ^-1 was produced with a conversion yield of 87%.

Original language	English
Pages (from-to)	677-682
Number of pages	6
Journal	Biotechnology Letters
Volume	34
Issue number	4
DOIs	https://doi.org/10.1007/s10529-011-0811-5
Publication status	Published - 2012 Apr 1

Keywords

Aryl acylamidase
Bioconversion
p-Acetaminophenol
Whole-cell catalysis

ASJC Scopus subject areas

Biotechnology

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10.1007/s10529-011-0811-5

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Ko, H. J., Bang, W. G., Kim, K. H., & Choi, I-G. (2012). Production of p-acetaminophenol by whole-cell catalysis using Escherichia coli overexpressing bacterial aryl acylamidase. Biotechnology Letters, 34(4), 677-682. https://doi.org/10.1007/s10529-011-0811-5

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abstract = "Aryl acylamidase (EC 3. 5. 1. 13, AAA) acts on the amide bond between aryl and acyl groups. Whole cells of Escherichia coli overexpressing a novel bacterial AAA synthesized p-acetaminophenol (p-AAP) from p-aminophenol (p-AP, aryl compound) and acetate (acyl donor). Optimum conditions were pH 5.5 and 35°C with 100 mM p-AP and 600 mM sodium acetate in 100 mM sodium phosphate buffer including 1% (v/v) Triton X-100 for 60 h. 13.1 g p-AAP l -1 was produced with a conversion yield of 87%.",

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