Production of p-acetaminophenol by whole-cell catalysis using Escherichia coli overexpressing bacterial aryl acylamidase

Hyeok Jin Ko, Won Gi Bang, Kyoung Heon Kim, In-Geol Choi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aryl acylamidase (EC 3. 5. 1. 13, AAA) acts on the amide bond between aryl and acyl groups. Whole cells of Escherichia coli overexpressing a novel bacterial AAA synthesized p-acetaminophenol (p-AAP) from p-aminophenol (p-AP, aryl compound) and acetate (acyl donor). Optimum conditions were pH 5.5 and 35°C with 100 mM p-AP and 600 mM sodium acetate in 100 mM sodium phosphate buffer including 1% (v/v) Triton X-100 for 60 h. 13.1 g p-AAP l -1 was produced with a conversion yield of 87%.

Original languageEnglish
Pages (from-to)677-682
Number of pages6
JournalBiotechnology Letters
Volume34
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1

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Keywords

  • Aryl acylamidase
  • Bioconversion
  • p-Acetaminophenol
  • Whole-cell catalysis

ASJC Scopus subject areas

  • Biotechnology

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