Abstract
Aryl acylamidase (EC 3. 5. 1. 13, AAA) acts on the amide bond between aryl and acyl groups. Whole cells of Escherichia coli overexpressing a novel bacterial AAA synthesized p-acetaminophenol (p-AAP) from p-aminophenol (p-AP, aryl compound) and acetate (acyl donor). Optimum conditions were pH 5.5 and 35°C with 100 mM p-AP and 600 mM sodium acetate in 100 mM sodium phosphate buffer including 1% (v/v) Triton X-100 for 60 h. 13.1 g p-AAP l -1 was produced with a conversion yield of 87%.
Original language | English |
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Pages (from-to) | 677-682 |
Number of pages | 6 |
Journal | Biotechnology letters |
Volume | 34 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2012 Apr |
Keywords
- Aryl acylamidase
- Bioconversion
- Whole-cell catalysis
- p-Acetaminophenol
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology