Production of poly-β-hydroxybutyrate by Alcaligenes eutrophus transformants harbouring cloned phbCAB genes

J. S. Park, Hae Chul Park, T. L. Huh, Y. H. Lee

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Three transformants of Alcaligenes eutrophus harbouring the recombinant plasmids containing phbCAB, phbAB, and phbC genes, were cultivated to investigate the effect of cloned genes on cell growth and poly-β-hydroxybutyrate accumulation. Both in the nutrient-rich and minimal media, the increased PHB accumulation in the transformants was observed compared to the parent strain, and this was the result of the increased enzyme activities in the transformants. Low carbon concentration and high C/N molar ratio favored higher PHB accumulations in the transformants. The transformant harbouring the phbC gene showed the highest PHB accumulation, which indicated that PHB synthase was the most critical enzyme for PHB biosynthesis in the transformant.

Original languageEnglish
Pages (from-to)735-740
Number of pages6
JournalBiotechnology Letters
Volume17
Issue number7
DOIs
Publication statusPublished - 1995 Aug 22
Externally publishedYes

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Hydroxybutyrates
Alcaligenes
Genes
Biosynthesis
Enzyme activity
Cell growth
Enzymes
Nutrients
Plasmids
Carbon
Food
Growth

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Production of poly-β-hydroxybutyrate by Alcaligenes eutrophus transformants harbouring cloned phbCAB genes. / Park, J. S.; Park, Hae Chul; Huh, T. L.; Lee, Y. H.

In: Biotechnology Letters, Vol. 17, No. 7, 22.08.1995, p. 735-740.

Research output: Contribution to journalArticle

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