Production of protein microarrays for cell culture using electrostatic deposition

Hyuneui Lim, Dongsoo Kim, Wonhee Lee, Yongdoo Park, Kyuback Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We used the electrostatic deposition (ESD) method to prepare the protein microarray for observation of the stem cell responses to pattern size, space and shapes. The ESD method allows a reduction in spot size, high efficiency of substance transfer, and high rate in fabrication as a result of ability to simultaneously deposit thousands of identical spots. Typical electrospraying conditions for the deposition of proteins were a voltage of 3.5-4.5 keV and the humidity under 40%. The patterns of masks have a variety of shapes, spaces, and hole sizes from 10 to 300 μm. Collagen is deposited on conducting polymer in a dry state, preserving the functional activity of proteins. The surface morphology of deposited spots was observed using an atomic force microscope and a scanning electron microscope. Human mesenchymal stem cells were cultured on each extracelluar protein patterned sample and cellular morphology was observed by light microscopy. Cell attachment and spreading on the surface are dependent on the deposition conditions by ESD. Protein deposition with short time is effective on the cell culture. However, collagen pattern size, space and shapes in micrometer size seemed to have little effect on cellular response in our design.

Original languageEnglish
Pages (from-to)124-130
Number of pages7
JournalSensors and Actuators, A: Physical
Volume139
Issue number1-2 SPEC. ISS.
DOIs
Publication statusPublished - 2007 Sep 12

Keywords

  • Cell culture
  • ESD
  • Protein microarray
  • Stencil mask

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering

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