Abstract
We used the electrostatic deposition (ESD) method to prepare the protein microarray for observation of the stem cell responses to pattern size, space and shapes. The ESD method allows a reduction in spot size, high efficiency of substance transfer, and high rate in fabrication as a result of ability to simultaneously deposit thousands of identical spots. Typical electrospraying conditions for the deposition of proteins were a voltage of 3.5-4.5 keV and the humidity under 40%. The patterns of masks have a variety of shapes, spaces, and hole sizes from 10 to 300 μm. Collagen is deposited on conducting polymer in a dry state, preserving the functional activity of proteins. The surface morphology of deposited spots was observed using an atomic force microscope and a scanning electron microscope. Human mesenchymal stem cells were cultured on each extracelluar protein patterned sample and cellular morphology was observed by light microscopy. Cell attachment and spreading on the surface are dependent on the deposition conditions by ESD. Protein deposition with short time is effective on the cell culture. However, collagen pattern size, space and shapes in micrometer size seemed to have little effect on cellular response in our design.
| Original language | English |
|---|---|
| Pages (from-to) | 124-130 |
| Number of pages | 7 |
| Journal | Sensors and Actuators, A: Physical |
| Volume | 139 |
| Issue number | 1-2 SPEC. ISS. |
| DOIs | |
| Publication status | Published - 2007 Sep 12 |
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Keywords
- Cell culture
- ESD
- Protein microarray
- Stencil mask
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Mechanical Engineering
- Instrumentation
Cite this
Production of protein microarrays for cell culture using electrostatic deposition. / Lim, Hyuneui; Kim, Dongsoo; Lee, Wonhee; Park, Yongdoo; Lee, Kyu Back.
In: Sensors and Actuators, A: Physical, Vol. 139, No. 1-2 SPEC. ISS., 12.09.2007, p. 124-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of protein microarrays for cell culture using electrostatic deposition
AU - Lim, Hyuneui
AU - Kim, Dongsoo
AU - Lee, Wonhee
AU - Park, Yongdoo
AU - Lee, Kyu Back
PY - 2007/9/12
Y1 - 2007/9/12
N2 - We used the electrostatic deposition (ESD) method to prepare the protein microarray for observation of the stem cell responses to pattern size, space and shapes. The ESD method allows a reduction in spot size, high efficiency of substance transfer, and high rate in fabrication as a result of ability to simultaneously deposit thousands of identical spots. Typical electrospraying conditions for the deposition of proteins were a voltage of 3.5-4.5 keV and the humidity under 40%. The patterns of masks have a variety of shapes, spaces, and hole sizes from 10 to 300 μm. Collagen is deposited on conducting polymer in a dry state, preserving the functional activity of proteins. The surface morphology of deposited spots was observed using an atomic force microscope and a scanning electron microscope. Human mesenchymal stem cells were cultured on each extracelluar protein patterned sample and cellular morphology was observed by light microscopy. Cell attachment and spreading on the surface are dependent on the deposition conditions by ESD. Protein deposition with short time is effective on the cell culture. However, collagen pattern size, space and shapes in micrometer size seemed to have little effect on cellular response in our design.
AB - We used the electrostatic deposition (ESD) method to prepare the protein microarray for observation of the stem cell responses to pattern size, space and shapes. The ESD method allows a reduction in spot size, high efficiency of substance transfer, and high rate in fabrication as a result of ability to simultaneously deposit thousands of identical spots. Typical electrospraying conditions for the deposition of proteins were a voltage of 3.5-4.5 keV and the humidity under 40%. The patterns of masks have a variety of shapes, spaces, and hole sizes from 10 to 300 μm. Collagen is deposited on conducting polymer in a dry state, preserving the functional activity of proteins. The surface morphology of deposited spots was observed using an atomic force microscope and a scanning electron microscope. Human mesenchymal stem cells were cultured on each extracelluar protein patterned sample and cellular morphology was observed by light microscopy. Cell attachment and spreading on the surface are dependent on the deposition conditions by ESD. Protein deposition with short time is effective on the cell culture. However, collagen pattern size, space and shapes in micrometer size seemed to have little effect on cellular response in our design.
KW - Cell culture
KW - ESD
KW - Protein microarray
KW - Stencil mask
UR - http://www.scopus.com/inward/record.url?scp=34548156804&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548156804&partnerID=8YFLogxK
U2 - 10.1016/j.sna.2007.04.066
DO - 10.1016/j.sna.2007.04.066
M3 - Article
AN - SCOPUS:34548156804
VL - 139
SP - 124
EP - 130
JO - Sensors and Actuators, A: Physical
JF - Sensors and Actuators, A: Physical
SN - 0924-4247
IS - 1-2 SPEC. ISS.
ER -