Production of recombinant human granulocyte-colony-stimulating factor in high cell density yeast cultures

Doo Suk Yang, Cheon Soon Bae, Jeewon Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The recombinant human granulocyte-colony-stimulating factor (rhG-CSF) was synthesized in a fusion protein using a GAL1-10 UAS in recombinant Saccharomyces cerevisiae and the intracellular KEX2 cleavage led excretion of mature rhG-CSF into the extracellular culture broth. The recombinant yeast growth in fed-batch cultures was controlled by precise computer-aided medium feed. The optimal C/N ratio in preinduction (glucose/Casamino acids) and post-induction (galactose/yeast extract) feed media was determined at 3 and 2, respectively. The final rhG-CSF and cell concentration was more than 60 mg/L and 70 g/L, respectively, with around 90% plasmid stability and negligible ethanol accumulation. Comparing the cell growth between the hG-CSF+ and hG-CSF- recombinant strains shows that the cloned gene product does not hamper the host cell growth.

Original languageEnglish
Pages (from-to)655-659
Number of pages5
JournalBiotechnology Letters
Volume19
Issue number7
DOIs
Publication statusPublished - 1997
Externally publishedYes

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Granulocyte Colony-Stimulating Factor
Yeast
Cell Count
Yeasts
Cell growth
Growth
Batch Cell Culture Techniques
Galactose
Glucose
Saccharomyces cerevisiae
Plasmids
Ethanol
Fusion reactions
Genes
Proteins
Acids

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Production of recombinant human granulocyte-colony-stimulating factor in high cell density yeast cultures. / Yang, Doo Suk; Bae, Cheon Soon; Lee, Jeewon.

In: Biotechnology Letters, Vol. 19, No. 7, 1997, p. 655-659.

Research output: Contribution to journalArticle

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