Production of (S)-3-hydroxybutyrate by metabolically engineered Saccharomyces cerevisiae

Eun Ju Yun, Suryang Kwak, Soo Rin Kim, Yong Cheol Park, Yong Su Jin, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

(S)-3-Hydroxybutyrate (S-3HB) can be used as a precursor for the synthesis of biodegradable polymers such as polyhydroxyalkanoate and stereo-specific fine chemicals such as antibiotics, pheromones, and drugs. For the production of S-3HB in yeast, the biosynthetic pathway of S-3HB from acetyl-CoA, consisting of the three enzymes, acetyl-CoA C-acetyltransferase (ACCT), acetoacetyl-CoA reductase (ACR), and 3-hydroxybutyryl-CoA thioesterase (HBT), was introduced into Saccharomyces cerevisiae. An engineered yeast strain overexpressing ERG10, hbd, and tesB genes not only exhibited enzyme activities of AACT, ACR, and HBT, but also produced S-3HB from ethanol. In order to increase the titer of S-3HB, a fed-batch fermentation based on pulse feeding of ethanol as a carbon source was performed, and a final S-3HB titer of 12.0. g/L was achieved. This is the first report on the production of 3HB by engineered yeast, utilizing ethanol as the carbon source, suggesting that the industrially preferred S. cerevisiae can be a promising host for producing S-3HB.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalJournal of Biotechnology
Volume209
DOIs
Publication statusPublished - 2015 Sep 1

Keywords

  • (S)-3-hydroxybutyrate
  • 3-Hydroxybutyryl-CoA thioesterase
  • Acetoacetyl-CoA reductase
  • Acetyl-CoA C-acetyltransferase
  • Polyhydroxyalkanoate
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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