Production of stearidonic acid-rich triacylglycerol via a two-step enzymatic esterification

Nam Ho Kim, Heejin Kim, Nakyung Choi, Yangha Kim, Byung Hee Kim, In-Hwan Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to synthesize stearidonic acid (SDA)-rich triacylglycerol (TAG) via a two-step lipase-catalyzed esterification under vacuum. SDA-rich fatty acid, which was prepared from echium oil via Candida rugosa lipase-catalyzed selective esterification, was used as the substrate. Two different immobilized lipases, Novozym 435 from Candida antarctica and Lipozyme TL IM from Thermomyces lanuginosus, were employed for the synthesis of SDA-rich TAG. In the first step, Novozym 435-catalyzed esterification of the SDA-rich fatty acid with glycerol was carried out for 2 h. In the second step, Lipozyme TL IM-catalyzed esterification of the reaction mixture from the first step was performed for an additional 10 h. The optimal reaction conditions for the second step were a temperature of 65 °C, an enzyme loading of 20%, and a vacuum of 0.7 kPa. Consequently, the maximum TAG conversion of ca. 86.4 wt% was obtained after 12 h via a two-step lipase-catalyzed esterification.

Original languageEnglish
Pages (from-to)332-337
Number of pages6
JournalFood Chemistry
Volume270
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Esterification
esterification
Triglycerides
Lipase
triacylglycerols
acids
Candida
Vacuum
Echium
Fatty Acids
Thermomyces lanuginosus
Candida rugosa
Pseudozyma antarctica
fatty acids
Glycerol
glycerol
Oils
stearidonic acid
oils
Temperature

Keywords

  • Esterification
  • Lipase
  • Lipozyme TL IM
  • Novozym 435
  • Stearidonic acid
  • Triacylglycerol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Cite this

Production of stearidonic acid-rich triacylglycerol via a two-step enzymatic esterification. / Kim, Nam Ho; Kim, Heejin; Choi, Nakyung; Kim, Yangha; Kim, Byung Hee; Kim, In-Hwan.

In: Food Chemistry, Vol. 270, 01.01.2019, p. 332-337.

Research output: Contribution to journalArticle

Kim, Nam Ho ; Kim, Heejin ; Choi, Nakyung ; Kim, Yangha ; Kim, Byung Hee ; Kim, In-Hwan. / Production of stearidonic acid-rich triacylglycerol via a two-step enzymatic esterification. In: Food Chemistry. 2019 ; Vol. 270. pp. 332-337.
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