Production of xylanase from a novel engineered Pichia pastoris and application to enzymatic hydrolysis process for biorefinery

Chan Kyum Kim, Han Suk Choi, Sang Jun Lee, Ja Hyun Lee, Ju Hun Lee, Hah Young Yoo, Sung Ok Han, Seung Wook Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, the xylanase gene from Cellulomonas flavigena KCTC 9104 was cloned into pPICZαB and expressed in Pichia pastoris X-33. An extracellular endo-1,4-β-xylanase was produced by novel engineered P. pastoris (rXynCf) and purified by Ni-NTA affinity column. Characterization of rXynCf was performed and results are as follows: 38kDa molecular weight, 55°C optimum temperature and optimum pH of 6. Under the conditions, the Km and V max of rXynCf were 3.6±0.08mg/mL and 4505±52μmol/minmg, respectively. rXynCf was applied in enzymatic hydrolysis process for sugars production from lignocellulosic biomass. Empty fruit bunch (EFB) was selected as a feedstock, and the total sugars conversion was found to be 3.8%, meanwhile the conversion by alkaline pretreatment improved approximately 16-fold (61.1%). In addition, rXynCf shows similar xylose conversion to commercial xylanase. Therefore, due to its properties, rXynCf has considerable potential in biorefinery applications.

Original languageEnglish
JournalProcess Biochemistry
DOIs
Publication statusAccepted/In press - 2017

Keywords

  • Biorefinery
  • Cellulomonas flavigena
  • Enzymatic hydrolysis
  • Pichia pastoris
  • Xylanase

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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