Profilin 2 promotes migration, invasion, and stemness of HT29 human colorectal cancer stem cells

Min Jung Kim, Yoo Sun Lee, Gi Yeon Han, Han Na Lee, Chiyoung Ahn, Chan Wha Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We investigated the role of profilin 2 in the stemness, migration, and invasion of HT29 cancer stem cells (CSCs). Increased and decreased levels of profilin 2 significantly enhanced and suppressed the self-renewal, migration, and invasion ability of HT29 CSCs, respectively. Moreover, profilin 2 directly regulated the expression of stemness markers (CD133, SOX2, and β-catenin) and epithelial mesenchymal transition (EMT) markers (E-cadherin and snail). CD133 and β-catenin were up-regulated by overexpression of profilin 2 and down-regulated by depletion of profilin 2. SOX2 was decreased by profilin 2 depletion. E-cadherin was not influenced by profilin 2- overexpression but increased by profilin 2- knockdown. The expression of snail was suppressed by profilin 2- knockdown. We speculated that stemness and the EMT are closely linked through profilin 2-related pathways. Therefore, this study indicates that profilin 2 affects the metastatic potential and stemness of colorectal CSCs by regulating EMT- And stemness-related proteins.

Original languageEnglish
Pages (from-to)1438-1446
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume79
Issue number9
DOIs
Publication statusPublished - 2015

Keywords

  • Cancer stem cells (CSCs)
  • Invasion
  • Migration
  • Profilin 2
  • Stemness

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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