Progesterone regulates FGF10, MET, IGFBP1, and IGFBP3 in the endometrium of the ovine uterus

M. Carey Satterfield, Kanako Hayashi, Gwonhwa Song, Sarah G. Black, Fuller W. Bazer, Thomas E. Spencer

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Progesterone (P4) is unequivocally required to maintain a uterine environment conducive to pregnancy. This study investigated the effects of P4 treatment on expression of selected growth factors (fibroblast growth factor 7 [FGF7], FGF10, hepatocyte growth factor [HGF], and insulin-like growth factors [IGF1 and IGF2]), their receptors (MET, FGFR2(IIIB), and IGF1R), and IGF binding proteins (IGFBPs) in the ovine uterus. Ewes received daily injections of corn oil vehicle (CO) or 25 mg of P4 in vehicle from 36 h after mating (Day 0) to hysterectomy on Day 9 or Day 12. Another group received P4 to Day 8 and 75 mg of mifepristone (RU486, a P4 receptor antagonist) from Day 8 through Day 12. Endometrial FGF10 mRNA levels increased between Day 9 and Day 12 and in response to P4 on Day 9 in CO-treated ewes, which had larger blastocysts, and were substantially reduced in P4+RU486-treated ewes, which had no blastocysts on Day 12. Endometrial FGF7 or HGF mRNA levels were not affected by day or reduced by RU486 treatment, but MET mRNA levels were higher in P4-treated ewes on Day 9 and Day 12. Levels of IGF1, IGF2, and IGF1R mRNA in the endometria were not affected by early P4 treatment. Although stromal IGFBPs were unaffected by P4, levels of IGFBP1 and IGFBP3 mRNA in uterine luminal epithelia were increased substantially between Day 9 and Day 12 of pregnancy in CO-treated ewes and on Day 9 in early P4-treated ewes. Therefore, FGF10, MET, IGFBP1, and IGFBP3 are P4-regulated factors within the endometrium of the ovine uterus that have potential effects on endometrial function and peri-implantation blastocyst growth and development.

Original languageEnglish
Pages (from-to)1226-1236
Number of pages11
JournalBiology of Reproduction
Volume79
Issue number6
DOIs
Publication statusPublished - 2008 Dec 1
Externally publishedYes

Fingerprint

Endometrium
Uterus
Progesterone
Sheep
Corn Oil
Messenger RNA
Fibroblast Growth Factor 7
Insulin-Like Growth Factor Binding Proteins
Hepatocyte Growth Factor
Blastocyst
Mifepristone
Pregnancy
Somatomedins
Hysterectomy
Growth and Development
Intercellular Signaling Peptides and Proteins
Therapeutics
Epithelium
Injections

Keywords

  • Early development
  • Growth factors
  • Pregnancy
  • Progesterone
  • Uterus

ASJC Scopus subject areas

  • Cell Biology

Cite this

Progesterone regulates FGF10, MET, IGFBP1, and IGFBP3 in the endometrium of the ovine uterus. / Satterfield, M. Carey; Hayashi, Kanako; Song, Gwonhwa; Black, Sarah G.; Bazer, Fuller W.; Spencer, Thomas E.

In: Biology of Reproduction, Vol. 79, No. 6, 01.12.2008, p. 1226-1236.

Research output: Contribution to journalArticle

Satterfield, M. Carey ; Hayashi, Kanako ; Song, Gwonhwa ; Black, Sarah G. ; Bazer, Fuller W. ; Spencer, Thomas E. / Progesterone regulates FGF10, MET, IGFBP1, and IGFBP3 in the endometrium of the ovine uterus. In: Biology of Reproduction. 2008 ; Vol. 79, No. 6. pp. 1226-1236.
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