Prognostic value of carbonic anhydrase IX and Ki-67 expression in squamous cell carcinoma of the tongue

Seok Jin Kim, Hye Jin Shin, Kwang-Yoon Jung, Seung-Kuk Baek, Bong Kyung Shin, Jung-Woo Choi, Byung Soo Kim, Sang Won Shin, Yeul Hong Kim, Jun Suk Kim, Egbert Oosterwijk

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Abstract

Background: Hypoxia-induced changes may allow tumor cells to survive under sustained hypoxic microenvironments resulting in achievement of more aggressive phenotypes. The purpose of this study is to determine the prognostic relevance of the expression of carbonic anhydrase IX (CA IX), a hypoxia-related protein in surgically resected squamous cell carcinoma of the tongue. We also relate CA IX to Ki-67 expression representing tumor cell proliferation to provide a prognostic model. Methods: We analysed the expression of CA IX and Ki-67 with immunohistochemistry in 60 patients with squamous cell carcinoma of the tongue. Results: The percentage of CA IX-positive tumor cells had a wide variation from 0.0 to 77.5%, and the Ki-67 expression was 1.50-75.1%. High CA IX and Ki-67 expression (≥10.0% of tumor cells positively stained with CA IX and Ki-67) was associated with a poorer overall survival (P < 0.05). High CA IX and Ki-67 expression showed shorter disease-free survival (DFS), although they are not statistically significant. To make a risk model based on the expression of CA IX and Ki-67, we divided the patients into three groups: high risk (high CA IX and Ki-67), low risk (low CA IX and Ki-67) and intermediate risk (either high CA IX or Ki-67). Being in the high-risk group was found to be an independent prognostic factor for overall survival and DFS in multivariate analysis (P < 0.05). Conclusion: The expression of CA IX and Ki-67 may be useful for predicting prognosis in squamous cell carcinoma of the tongue.

Original languageEnglish
Pages (from-to)812-819
Number of pages8
JournalJapanese Journal of Clinical Oncology
Volume37
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1

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Tongue
Squamous Cell Carcinoma
Disease-Free Survival
Carbonic Anhydrase IX
Neoplasms
Survival
Multivariate Analysis
Immunohistochemistry
Cell Proliferation
Phenotype

Keywords

  • Carbonic anhydrase IX
  • Hypoxia
  • Ki-67
  • Squamous cell carcinoma
  • Tongue

ASJC Scopus subject areas

  • Oncology

Cite this

@article{fa6e7c0f4410453192f87940472e699d,
title = "Prognostic value of carbonic anhydrase IX and Ki-67 expression in squamous cell carcinoma of the tongue",
abstract = "Background: Hypoxia-induced changes may allow tumor cells to survive under sustained hypoxic microenvironments resulting in achievement of more aggressive phenotypes. The purpose of this study is to determine the prognostic relevance of the expression of carbonic anhydrase IX (CA IX), a hypoxia-related protein in surgically resected squamous cell carcinoma of the tongue. We also relate CA IX to Ki-67 expression representing tumor cell proliferation to provide a prognostic model. Methods: We analysed the expression of CA IX and Ki-67 with immunohistochemistry in 60 patients with squamous cell carcinoma of the tongue. Results: The percentage of CA IX-positive tumor cells had a wide variation from 0.0 to 77.5{\%}, and the Ki-67 expression was 1.50-75.1{\%}. High CA IX and Ki-67 expression (≥10.0{\%} of tumor cells positively stained with CA IX and Ki-67) was associated with a poorer overall survival (P < 0.05). High CA IX and Ki-67 expression showed shorter disease-free survival (DFS), although they are not statistically significant. To make a risk model based on the expression of CA IX and Ki-67, we divided the patients into three groups: high risk (high CA IX and Ki-67), low risk (low CA IX and Ki-67) and intermediate risk (either high CA IX or Ki-67). Being in the high-risk group was found to be an independent prognostic factor for overall survival and DFS in multivariate analysis (P < 0.05). Conclusion: The expression of CA IX and Ki-67 may be useful for predicting prognosis in squamous cell carcinoma of the tongue.",
keywords = "Carbonic anhydrase IX, Hypoxia, Ki-67, Squamous cell carcinoma, Tongue",
author = "Kim, {Seok Jin} and Shin, {Hye Jin} and Kwang-Yoon Jung and Seung-Kuk Baek and Shin, {Bong Kyung} and Jung-Woo Choi and Kim, {Byung Soo} and Shin, {Sang Won} and Kim, {Yeul Hong} and Kim, {Jun Suk} and Egbert Oosterwijk",
year = "2007",
month = "11",
day = "1",
doi = "10.1093/jjco/hym121",
language = "English",
volume = "37",
pages = "812--819",
journal = "Japanese Journal of Clinical Oncology",
issn = "0368-2811",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - Prognostic value of carbonic anhydrase IX and Ki-67 expression in squamous cell carcinoma of the tongue

AU - Kim, Seok Jin

AU - Shin, Hye Jin

AU - Jung, Kwang-Yoon

AU - Baek, Seung-Kuk

AU - Shin, Bong Kyung

AU - Choi, Jung-Woo

AU - Kim, Byung Soo

AU - Shin, Sang Won

AU - Kim, Yeul Hong

AU - Kim, Jun Suk

AU - Oosterwijk, Egbert

PY - 2007/11/1

Y1 - 2007/11/1

N2 - Background: Hypoxia-induced changes may allow tumor cells to survive under sustained hypoxic microenvironments resulting in achievement of more aggressive phenotypes. The purpose of this study is to determine the prognostic relevance of the expression of carbonic anhydrase IX (CA IX), a hypoxia-related protein in surgically resected squamous cell carcinoma of the tongue. We also relate CA IX to Ki-67 expression representing tumor cell proliferation to provide a prognostic model. Methods: We analysed the expression of CA IX and Ki-67 with immunohistochemistry in 60 patients with squamous cell carcinoma of the tongue. Results: The percentage of CA IX-positive tumor cells had a wide variation from 0.0 to 77.5%, and the Ki-67 expression was 1.50-75.1%. High CA IX and Ki-67 expression (≥10.0% of tumor cells positively stained with CA IX and Ki-67) was associated with a poorer overall survival (P < 0.05). High CA IX and Ki-67 expression showed shorter disease-free survival (DFS), although they are not statistically significant. To make a risk model based on the expression of CA IX and Ki-67, we divided the patients into three groups: high risk (high CA IX and Ki-67), low risk (low CA IX and Ki-67) and intermediate risk (either high CA IX or Ki-67). Being in the high-risk group was found to be an independent prognostic factor for overall survival and DFS in multivariate analysis (P < 0.05). Conclusion: The expression of CA IX and Ki-67 may be useful for predicting prognosis in squamous cell carcinoma of the tongue.

AB - Background: Hypoxia-induced changes may allow tumor cells to survive under sustained hypoxic microenvironments resulting in achievement of more aggressive phenotypes. The purpose of this study is to determine the prognostic relevance of the expression of carbonic anhydrase IX (CA IX), a hypoxia-related protein in surgically resected squamous cell carcinoma of the tongue. We also relate CA IX to Ki-67 expression representing tumor cell proliferation to provide a prognostic model. Methods: We analysed the expression of CA IX and Ki-67 with immunohistochemistry in 60 patients with squamous cell carcinoma of the tongue. Results: The percentage of CA IX-positive tumor cells had a wide variation from 0.0 to 77.5%, and the Ki-67 expression was 1.50-75.1%. High CA IX and Ki-67 expression (≥10.0% of tumor cells positively stained with CA IX and Ki-67) was associated with a poorer overall survival (P < 0.05). High CA IX and Ki-67 expression showed shorter disease-free survival (DFS), although they are not statistically significant. To make a risk model based on the expression of CA IX and Ki-67, we divided the patients into three groups: high risk (high CA IX and Ki-67), low risk (low CA IX and Ki-67) and intermediate risk (either high CA IX or Ki-67). Being in the high-risk group was found to be an independent prognostic factor for overall survival and DFS in multivariate analysis (P < 0.05). Conclusion: The expression of CA IX and Ki-67 may be useful for predicting prognosis in squamous cell carcinoma of the tongue.

KW - Carbonic anhydrase IX

KW - Hypoxia

KW - Ki-67

KW - Squamous cell carcinoma

KW - Tongue

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U2 - 10.1093/jjco/hym121

DO - 10.1093/jjco/hym121

M3 - Article

VL - 37

SP - 812

EP - 819

JO - Japanese Journal of Clinical Oncology

JF - Japanese Journal of Clinical Oncology

SN - 0368-2811

IS - 11

ER -