Prolonged survival of mice with glioma injected intracerebrally with double cytokine-secreting cells

T. Lichtor, R. P. Glick, Tae Sung Kim, R. Hand, E. P. Cohen

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52 Citations (Scopus)

Abstract

A novel approach toward the treatment of glioma was developed in a murine model. The genes for both interleukin-2 (IL-2) and interferon-γ (IFN-γ) were first transfected into a mouse fibroblast cell line that expresses defined major histocompatibility complex (MHC) determinants (H-2(k)). The double cytokine-secreting cells were then cotransplanted intracerebrally with the G1261 murine glioma cell line into syngeneic C57BL/6 mice (H-2b) whose cells differed at the MHC from the cellular immunogen. The results indicate that the survival of mice with glioma injected with the cytokine-secreting allogeneic cells was significantly prolonged, relative to the survival of mice receiving equivalent numbers of glioma cells alone. Using a standard 51Cr-release assay, the specific release of isotope from labeled G1261 cells coincubated with spleen cells from mice injected intracerebrally with the glioma cells and the cytokine-secreting fibroblasts was significantly higher than the release of isotope from glioma cells coincubated with spleen cells from nonimmunized mice. The cellular antiglioma response was mediated by natural killer/lymphokine-activated killer and Lyt-2.2+ (CD8+) cells. The increased survival of mice with glioma and the specific immunocytotoxic responses after immunization with fibroblasts modified to secrete both IL-2 and IFN-γ indicate the potential of an immunotherapeutic approach to gliomas with cytokine-secreting cells.

Original languageEnglish
Pages (from-to)1038-1044
Number of pages7
JournalJournal of Neurosurgery
Volume83
Issue number6
Publication statusPublished - 1995 Dec 1
Externally publishedYes

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ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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