Promotion of skeletal muscle differentiation by K252a with tyrosine phosphorylation of focal adhesion: A possible involvement of small GTPase Rho

Kun Ho Lee, Seung Hye Lee, Daegun Kim, Sangmyung Rhee, Chungho Kim, Chin Ha Chung, Hyockman Kwon, Man Sik Kang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

K252a, a protein kinase inhibitor, acts as a neurotrophic factor in several neuronal cells. In this study we show that K252a enhanced the differentiation of C2C12 myoblasts as well as tyrosine phosphorylation of several focal adhesion-associated proteins including p130(Cas), focal adhesion kinase, and paxillin. The tyrosine phosphorylation of these proteins, reaching a maximum at 30 min after K252a treatment, closely correlated with the colocalization of these proteins in focal adhesion complexes and the coimmunoprecipitation of these proteins with p130(Cas). In addition, K252a stimulated longitudinal development of stress fiber-like structures and cell-matrix interaction in postmitotic myoblasts and eventually formation of well-developed myofibrils in multinucleated myotubes. Herbimycin A, a potent inhibitor of Src family kinases, and cytochalasin D, a selective disrupting-agent of actin filament, completely inhibited K252a- induced tyrosine phosphorylation as well as myoblast differentiation. Similar inhibitory effect was observed in the cells scrape loaded with a Rho inhibitor, C3 transferase, and the treatment of K252a induced a rapid translocation of Rho. These results are consistent with the model that Rho- dependent tyrosine phosphorylation of focal adhesion-associated proteins plays an important role in skeletal muscle differentiation.

Original languageEnglish
Pages (from-to)401-415
Number of pages15
JournalExperimental Cell Research
Volume252
Issue number2
DOIs
Publication statusPublished - 1999 Nov 1
Externally publishedYes

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Focal Adhesions
Monomeric GTP-Binding Proteins
Tyrosine
Skeletal Muscle
Phosphorylation
Myoblasts
Crk-Associated Substrate Protein
Paxillin
Cytochalasin D
Focal Adhesion Protein-Tyrosine Kinases
Stress Fibers
Proteins
src-Family Kinases
Myofibrils
Skeletal Muscle Fibers
Nerve Growth Factors
Protein Kinase Inhibitors
Transferases
Actin Cytoskeleton
Cell Communication

ASJC Scopus subject areas

  • Cell Biology

Cite this

Promotion of skeletal muscle differentiation by K252a with tyrosine phosphorylation of focal adhesion : A possible involvement of small GTPase Rho. / Lee, Kun Ho; Lee, Seung Hye; Kim, Daegun; Rhee, Sangmyung; Kim, Chungho; Chung, Chin Ha; Kwon, Hyockman; Kang, Man Sik.

In: Experimental Cell Research, Vol. 252, No. 2, 01.11.1999, p. 401-415.

Research output: Contribution to journalArticle

Lee, Kun Ho ; Lee, Seung Hye ; Kim, Daegun ; Rhee, Sangmyung ; Kim, Chungho ; Chung, Chin Ha ; Kwon, Hyockman ; Kang, Man Sik. / Promotion of skeletal muscle differentiation by K252a with tyrosine phosphorylation of focal adhesion : A possible involvement of small GTPase Rho. In: Experimental Cell Research. 1999 ; Vol. 252, No. 2. pp. 401-415.
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