Prophylactic efficacy of a new gentamicin-releasing urethral catheter in short-term catheterized rabbits

Y. H. Cho, S. J. Lee, J. Y. Lee, S. W. Kim, Ick Chan Kwon, S. Y. Chung, M. S. Yoon

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Abstract

Objective: To describe an indwelling urethral catheter coated with gentamicin sulphate on the inner and outer surface of the catheter, and to evaluate the efficacy and safety of this catheter in preventing catheter-associated infections in rabbits. Materials and methods: Sixty rabbits were divided equally into control and experimental groups which were then subdivided equally according to the duration of catheterization (1, 3 and 5 days). Silicone-treated latex catheters were used in the control group and gentamicin-releasing catheters in the experimental group. Urine samples and surface swabs from the catheter were cultured for bacteriological assessment, and the catheter surface examined by scanning electron microscopy to structurally analyse the biofilms. Results: The gentamicin-releasing catheter reduced the incidence of bacteriuria (defined as ≥ 100 c.f.u./mL) after both 3 and 5 days of catheterization (eight and 10 rabbits, respectively, for the control catheter, vs two and four rabbits for the gentamicin-releasing catheter, P<0.05). The surfaces of the gentamicin-releasing catheter were colonized less often than those of the control catheter after both 3 and 5 days (eight and 10, respectively, for the control, vs one and four for the gentamicin-releasing catheter. P<0.05). Scanning electron microscopy showed the formation of bacterial biofilm throughout the 3-day and 5-day control catheters, but deterioration of the bacterial biofilm was visible on the surface of the gentamicin-releasing catheters. Conclusion: This new gentamicin-releasing catheter produced an antibacterial barrier which inhibited catheter-associated urinary tract infection with no toxicity for at least 5 days. These in vivo studies suggest that this new catheter may be useful for controlling infection, with systemic and local safety, in patients undergoing short-term indwelling urethral catheterization.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalBJU International
Volume87
Issue number1
DOIs
Publication statusPublished - 2001 Feb 1
Externally publishedYes

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Keywords

  • Bacterial biofilm
  • Catheter-associated urinary tract infection
  • Gentamicin
  • Urethral catheter

ASJC Scopus subject areas

  • Urology

Cite this

Cho, Y. H., Lee, S. J., Lee, J. Y., Kim, S. W., Kwon, I. C., Chung, S. Y., & Yoon, M. S. (2001). Prophylactic efficacy of a new gentamicin-releasing urethral catheter in short-term catheterized rabbits. BJU International, 87(1), 104-109. https://doi.org/10.1046/j.1464-410X.2001.00978.x