Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats

Chul Sang Lee, Pei Lei Tan, Ju Young Eor, Da Hye Choi, Miri Park, Sung Keum Seo, Seokmin Yoon, Siyoung Yang, Sae Hun Kim

Research output: Contribution to journalArticle

Abstract

BACKGROUND: This study investigated the in vivo prophylactic effect of probiotic chocolate on constipation. Rats were administered chocolate containing 2.5 × 1010 CFU g−1 of probiotics daily for 4 weeks and treated with loperamide (5 mg kg−1) daily at the fourth week of treatment. RESULTS: Probiotic chocolate treatment significantly (P < 0.05) increased the intestinal motility, colon length, fecal moisture content and number of excreted fecal pellets in constipated rats. Moreover, quantitative real-time polymerase chain reaction data and histological images also revealed that both probiotic chocolate LYC and BB12 treatments were capable of upregulating the mRNA expression levels of colonic ZO-1, occludin and AQP8, leading to the maintenance of the defensive barrier function in the constipated rats compared with the negative controls. Interestingly, these treatments also modulated gut bacterial populations by increasing the abundance levels of Lactobacillus and Bifidobacterium, as well as reducing the abundance level of Enterobacteriaceae. CONCLUSION: The present study demonstrated that probiotic chocolate LYC and BB12 could potentially be used as alternative agents for prophylactic constipation.

Original languageEnglish
JournalJournal of the Science of Food and Agriculture
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Probiotics
chocolate
probiotics
rats
constipation
Constipation
Loperamide
Occludin
gastrointestinal motility
Gastrointestinal Motility
Bifidobacterium
Lactobacillus
Enterobacteriaceae
in vivo studies
colon
pellets
Real-Time Polymerase Chain Reaction
Colon
quantitative polymerase chain reaction
digestive system

Keywords

  • constipation
  • functional chocolate
  • gastrointestinal function
  • intestinal barrier function
  • lactic acid bacteria

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics

Cite this

Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats. / Lee, Chul Sang; Tan, Pei Lei; Eor, Ju Young; Choi, Da Hye; Park, Miri; Seo, Sung Keum; Yoon, Seokmin; Yang, Siyoung; Kim, Sae Hun.

In: Journal of the Science of Food and Agriculture, 01.01.2019.

Research output: Contribution to journalArticle

Lee, Chul Sang ; Tan, Pei Lei ; Eor, Ju Young ; Choi, Da Hye ; Park, Miri ; Seo, Sung Keum ; Yoon, Seokmin ; Yang, Siyoung ; Kim, Sae Hun. / Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats. In: Journal of the Science of Food and Agriculture. 2019.
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