TY - JOUR
T1 - Prospects for Bio-Industrial Application of an Extremely Alkaline Mannanase From Bacillus subtilis subsp. inaquosorum CSB31
AU - Regmi, Sudip
AU - Yoo, Hah Y.
AU - Choi, Yun H.
AU - Choi, Yoon S.
AU - Yoo, Jin C.
AU - Kim, Seung W.
N1 - Funding Information:
S.R. and H.Y.Y contributed equally to this work. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (MEST) (NRF-2015R1D1A1A01059483, NRF-2017R1D1A1B03030996) and the Bio-industry Technology Development Program, Ministry of Agriculture, Food and Rural Affairs (115073-2).
Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/11
Y1 - 2017/11
N2 - Mannan-degrading enzymes have been growing interest in bio-industrial applications, such as the pulp and paper, food, and pharmaceutical industries. In this study, an extremely alkaline mannanase (MnB31) is produced by Bacillus subtilis subsp. inaquosorum CSB31. MnB31 is purified to 17.92-fold with a 21.51% yield and specific activity of 1,796.13 U mg−1 by anion-exchange and gel filtration column chromatography. The biochemical characterization of MnB31 is performed, and the results are as follows: molecular weight of ≈47 kDa with an optimum temperature of 60 °C and pH of 12.5. The enzyme is strongly activated by Co2+, Mn2+, Na+, and K+, and inhibited by Zn2+, Ni2+, and Mg2+. Halo-tolerance (10% NaCl), urea stability (3 M), and protease resistance are also observed. The kinetic parameters of MnB31 are found to be Km of 0.043 mg ml−1, and Vmax of 1,046 ± 3.605 U mg−1, respectively. In addition, the thermodynamical parameters are investigated; the activation energy (Ea) is found to be 31.36 kJ mol−1 with a Kcat value of 156.9 × 104 s−1, ΔH (28.59 kJ mol−1), ΔG (42.38 kJ mol−1), ΔS (−41.39 J mol−1 K−1), Q10 (1.40), ΔGE–S (−8.697 kJ mol−1), and ΔGE-T (−48.22 kJ mol−1). These results suggest that MnB31 has potential bio-industrial application, due to its greater hydrolytic efficiency and feasibility of enzymatic reaction.
AB - Mannan-degrading enzymes have been growing interest in bio-industrial applications, such as the pulp and paper, food, and pharmaceutical industries. In this study, an extremely alkaline mannanase (MnB31) is produced by Bacillus subtilis subsp. inaquosorum CSB31. MnB31 is purified to 17.92-fold with a 21.51% yield and specific activity of 1,796.13 U mg−1 by anion-exchange and gel filtration column chromatography. The biochemical characterization of MnB31 is performed, and the results are as follows: molecular weight of ≈47 kDa with an optimum temperature of 60 °C and pH of 12.5. The enzyme is strongly activated by Co2+, Mn2+, Na+, and K+, and inhibited by Zn2+, Ni2+, and Mg2+. Halo-tolerance (10% NaCl), urea stability (3 M), and protease resistance are also observed. The kinetic parameters of MnB31 are found to be Km of 0.043 mg ml−1, and Vmax of 1,046 ± 3.605 U mg−1, respectively. In addition, the thermodynamical parameters are investigated; the activation energy (Ea) is found to be 31.36 kJ mol−1 with a Kcat value of 156.9 × 104 s−1, ΔH (28.59 kJ mol−1), ΔG (42.38 kJ mol−1), ΔS (−41.39 J mol−1 K−1), Q10 (1.40), ΔGE–S (−8.697 kJ mol−1), and ΔGE-T (−48.22 kJ mol−1). These results suggest that MnB31 has potential bio-industrial application, due to its greater hydrolytic efficiency and feasibility of enzymatic reaction.
KW - Bacillus subtilis
KW - bioindustry
KW - lignocellulose
KW - mannanase
KW - thermodynamic characterization
UR - http://www.scopus.com/inward/record.url?scp=85030107246&partnerID=8YFLogxK
U2 - 10.1002/biot.201700113
DO - 10.1002/biot.201700113
M3 - Article
C2 - 28843040
AN - SCOPUS:85030107246
VL - 12
JO - Biotechnology Journal
JF - Biotechnology Journal
SN - 1860-6768
IS - 11
M1 - 1700113
ER -