Protease-activated receptor-1 in human brain: Localization and functional expression in astrocytes

Candice E. Junge, C. Justin Lee, Katherine B. Hubbard, Zhoabin Zhang, Jeffrey J. Olson, John R. Hepler, Daniel J. Brat, Stephen F. Traynelis

Research output: Contribution to journalArticlepeer-review

116 Citations (Scopus)

Abstract

Protease-activated receptor-1 (PAR1) is a G-protein coupled receptor that is proteolytically activated by blood-derived serine proteases. Although PAR1 is best known for its role in coagulation and hemostasis, recent findings demonstrate that PAR1 activation has actions in the central nervous system (CNS) apart from its role in the vasculature. Rodent studies have demonstrated that PAR1 is expressed throughout the brain on neurons and astrocytes. PAR1 activation in vitro and in vivo appears to influence neurodegeneration and neuroprotection in animal models of stroke and brain injury. Because of increasing evidence that PAR1 has important and diverse roles in the CNS, we explored the protein localization and function of PAR1 in human brain. PAR1 is most intensely expressed in astrocytes of white and gray matter and moderately expressed in neurons. PAR1 and GFAP co-localization demonstrates that PAR1 is expressed on the cell body and on astrocytic endfeet that invest capillaries. PAR1 activation in the U178MG human glioblastoma cell line increased PI hydrolysis and intracellular Ca2+, indicating that PAR1 is functional in human glial-derived tumor cells. Primary cultures of human astrocytes and human glioblastoma cells respond to PAR1 activation by increasing intracellular Ca2+. Together, these results demonstrate that PAR1 is expressed in human brain and functional in glial tumors and cultures derived from it. Because serine proteases may enter brain tissue and activate PAR1 when the blood brain barrier (BBB) breaks down, pharmacological manipulation of PAR1 signaling may provide a potential therapeutic target for neuroprotection in human neurological disorders.

Original languageEnglish
Pages (from-to)94-103
Number of pages10
JournalExperimental Neurology
Volume188
Issue number1
DOIs
Publication statusPublished - 2004 Jul

Keywords

  • Astrocyte
  • G-protein coupled receptor
  • Glioblastoma
  • PAR1
  • Protease-activated receptor
  • Serine protease
  • Thrombin

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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