Protease activated receptor 1-induced glutamate release in cultured astrocytes is mediated by Bestrophin-1 channel but not by vesicular exocytosis

Soo Jin Oh, Kyung Seok Han, Hyungju Park, Dong Ho Woo, Hye Yun Kim, Stephen F. Traynelis, Changjoon Lee

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background: Glutamate is the major transmitter that mediates the principal form of excitatory synaptic transmission in the brain. It has been well established that glutamate is released via Ca2+-dependent exocytosis of glutamate-containing vesicles in neurons. However, whether astrocytes exocytose to release glutamate under physiological condition is still unclear. Findings. We report a novel form of glutamate release in astrocytes via the recently characterized Ca2+-activated anion channel, Bestrophin-1 (Best1) by Ca2+ dependent mechanism through the channel pore. We demonstrate that upon activation of protease activated receptor 1 (PAR1), an increase in intracellular Ca2+ concentration leads to an opening of Best1 channels and subsequent release of glutamate in cultured astrocytes. Conclusions: These results provide strong molecular evidence for potential astrocyte-neuron interaction via Best1-mediated glutamate release.

Original languageEnglish
Article number38
JournalMolecular Brain
Volume5
Issue number1
DOIs
Publication statusPublished - 2012 Oct 16
Externally publishedYes

Fingerprint

PAR-1 Receptor
Exocytosis
Astrocytes
Glutamic Acid
Neurons
Synaptic Transmission
Anions
Brain

Keywords

  • Astrocyte
  • Bestrophin-1
  • Glutamate

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Molecular Biology

Cite this

Protease activated receptor 1-induced glutamate release in cultured astrocytes is mediated by Bestrophin-1 channel but not by vesicular exocytosis. / Oh, Soo Jin; Han, Kyung Seok; Park, Hyungju; Woo, Dong Ho; Kim, Hye Yun; Traynelis, Stephen F.; Lee, Changjoon.

In: Molecular Brain, Vol. 5, No. 1, 38, 16.10.2012.

Research output: Contribution to journalArticle

Oh, Soo Jin ; Han, Kyung Seok ; Park, Hyungju ; Woo, Dong Ho ; Kim, Hye Yun ; Traynelis, Stephen F. ; Lee, Changjoon. / Protease activated receptor 1-induced glutamate release in cultured astrocytes is mediated by Bestrophin-1 channel but not by vesicular exocytosis. In: Molecular Brain. 2012 ; Vol. 5, No. 1.
@article{4924ce5d981b4ee78becd8b57a0be644,
title = "Protease activated receptor 1-induced glutamate release in cultured astrocytes is mediated by Bestrophin-1 channel but not by vesicular exocytosis",
abstract = "Background: Glutamate is the major transmitter that mediates the principal form of excitatory synaptic transmission in the brain. It has been well established that glutamate is released via Ca2+-dependent exocytosis of glutamate-containing vesicles in neurons. However, whether astrocytes exocytose to release glutamate under physiological condition is still unclear. Findings. We report a novel form of glutamate release in astrocytes via the recently characterized Ca2+-activated anion channel, Bestrophin-1 (Best1) by Ca2+ dependent mechanism through the channel pore. We demonstrate that upon activation of protease activated receptor 1 (PAR1), an increase in intracellular Ca2+ concentration leads to an opening of Best1 channels and subsequent release of glutamate in cultured astrocytes. Conclusions: These results provide strong molecular evidence for potential astrocyte-neuron interaction via Best1-mediated glutamate release.",
keywords = "Astrocyte, Bestrophin-1, Glutamate",
author = "Oh, {Soo Jin} and Han, {Kyung Seok} and Hyungju Park and Woo, {Dong Ho} and Kim, {Hye Yun} and Traynelis, {Stephen F.} and Changjoon Lee",
year = "2012",
month = "10",
day = "16",
doi = "10.1186/1756-6606-5-38",
language = "English",
volume = "5",
journal = "Molecular Brain",
issn = "1756-6606",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Protease activated receptor 1-induced glutamate release in cultured astrocytes is mediated by Bestrophin-1 channel but not by vesicular exocytosis

AU - Oh, Soo Jin

AU - Han, Kyung Seok

AU - Park, Hyungju

AU - Woo, Dong Ho

AU - Kim, Hye Yun

AU - Traynelis, Stephen F.

AU - Lee, Changjoon

PY - 2012/10/16

Y1 - 2012/10/16

N2 - Background: Glutamate is the major transmitter that mediates the principal form of excitatory synaptic transmission in the brain. It has been well established that glutamate is released via Ca2+-dependent exocytosis of glutamate-containing vesicles in neurons. However, whether astrocytes exocytose to release glutamate under physiological condition is still unclear. Findings. We report a novel form of glutamate release in astrocytes via the recently characterized Ca2+-activated anion channel, Bestrophin-1 (Best1) by Ca2+ dependent mechanism through the channel pore. We demonstrate that upon activation of protease activated receptor 1 (PAR1), an increase in intracellular Ca2+ concentration leads to an opening of Best1 channels and subsequent release of glutamate in cultured astrocytes. Conclusions: These results provide strong molecular evidence for potential astrocyte-neuron interaction via Best1-mediated glutamate release.

AB - Background: Glutamate is the major transmitter that mediates the principal form of excitatory synaptic transmission in the brain. It has been well established that glutamate is released via Ca2+-dependent exocytosis of glutamate-containing vesicles in neurons. However, whether astrocytes exocytose to release glutamate under physiological condition is still unclear. Findings. We report a novel form of glutamate release in astrocytes via the recently characterized Ca2+-activated anion channel, Bestrophin-1 (Best1) by Ca2+ dependent mechanism through the channel pore. We demonstrate that upon activation of protease activated receptor 1 (PAR1), an increase in intracellular Ca2+ concentration leads to an opening of Best1 channels and subsequent release of glutamate in cultured astrocytes. Conclusions: These results provide strong molecular evidence for potential astrocyte-neuron interaction via Best1-mediated glutamate release.

KW - Astrocyte

KW - Bestrophin-1

KW - Glutamate

UR - http://www.scopus.com/inward/record.url?scp=84867318753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867318753&partnerID=8YFLogxK

U2 - 10.1186/1756-6606-5-38

DO - 10.1186/1756-6606-5-38

M3 - Article

C2 - 23062602

AN - SCOPUS:84867318753

VL - 5

JO - Molecular Brain

JF - Molecular Brain

SN - 1756-6606

IS - 1

M1 - 38

ER -