Protease-containing silicates as active antifouling materials

Jungbae Kim, Ray Delio, Jonathan S. Dordick

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27 Citations (Scopus)

Abstract

Biocatalytic silicates, composite materials composed of α-chymotrypsin and a silicate prepolymer, were prepared via a two-step polymerization process following solubilization of the enzyme in the polymerization media. This new approach resulted in active and stable composites, and a calculated half-life of over 350 days in aqueous buffer at 30°C. The high stability and activity of this biocatalytic silicate was likely due to the covalent attachment between α-chymotrypsin and the silicate matrix. The protease-containing silicate was resistant to fouling by nonselective protein binding, as demonstrated by the dramatically reduced binding of human serum albumin to the silicate material when compared to that of a silicate containing pre-inactivated α-chymotrypsin.

Original languageEnglish
Pages (from-to)551-555
Number of pages5
JournalBiotechnology Progress
Volume18
Issue number3
DOIs
Publication statusPublished - 2002 Jun 24
Externally publishedYes

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ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

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