Protection against diesel oil toxicity by sodium chloride-induced exopolysaccharides in Acinetobacter sp. strain DR1

Yoon Suk Kang, Woojun Park

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Acinetobacter sp. strain DR1 is capable of growth on diesel oil. Interestingly, the degradation of diesel oil by the strain DR1 is enhanced in the presence of sodium chloride (NaCl). However, the growth rate of strain DR1 is not affected by the presence of NaCl. Northern blot analysis has also demonstrated that the effect of NaCl on the degradation of diesel oil is not attributable to increased levels of alkane hydroxylase (AlkM-type) gene expression. Rather, we have noted an increase in the exopolysaccharide (EPS) yields of strain DR1 under high NaCl conditions (9-fold). The lag-time of diesel oil biodegradation was significantly shorter in the strain DR1 with exogenous EPS than in the controls, although EPS alone does not support the growth of strain DR1. The recovery of strain DR1 when exposed to diesel oil was accelerated when exogenous EPS was added to the medium. The overproduction of EPS was also noted in the presence of diesel oil and n-hexadecane. The data indicated that EPS overproduction might play a protective role against diesel oil toxicity. Along with the results of the soil microcosm tests, the data presented herein demonstrated that NaCl-induced EPS is associated with a reduction in diesel oil toxicity, and thus increases diesel oil biodegradation in Acinetobacter sp. strain DR1.

Original languageEnglish
Pages (from-to)118-123
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume109
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1

Fingerprint

Acinetobacter
Sodium chloride
Sodium Chloride
Toxicity
Oils
Biodegradation
Growth
Cytochrome P-450 CYP4A
Degradation
Gene expression
Northern Blotting
Soil
Soils
Gene Expression
Recovery

Keywords

  • Aliphatic hydrocarbon
  • Alkane
  • Bacteria
  • Biodegradation
  • Bioremediation
  • Soil bacterium
  • Total petroleum hydrocarbon

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Protection against diesel oil toxicity by sodium chloride-induced exopolysaccharides in Acinetobacter sp. strain DR1. / Kang, Yoon Suk; Park, Woojun.

In: Journal of Bioscience and Bioengineering, Vol. 109, No. 2, 01.02.2010, p. 118-123.

Research output: Contribution to journalArticle

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