Protection against kainate neurotoxicity by pyrrolidine dithiocarbamate

Eun Joo Shin, Jin Hyeong Jhoo, Won-Ki Kim, Wang Kee Jhoo, Chaeyoung Lee, Bae Dong Jung, Hyoung Chun Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

1. The effect of pyrrolidine dithiocarbamate (PDTC) on kainate (KA)-induced neurotoxicity was examined in Sprague-Dawley rats. 2. At 10 mg/kg, i.p., KA produced seizures accompanied by neuronal loss in the hippocampus and increased levels of malondialdehyde (MDA) and protein carbonyl. 3. Pretreatment with PDTC (100 or 200 mg/kg, p.o., every 12 h x5) blocked KA-induced neurotoxicities (seizures, increases in MDA and protein carbonyl and neuronal losses) in a dose-dependent manner. These effects were counteracted by the adenosine A 1 receptor antagonist 8-cyclopentyl-1,3-dimetliylxanthine (25 or 50 μg/kg, i.p.), but not by the A2A receptor antagonist 1,3,7-trimethyl-8-(3-chlorostyryl)xanthine (0.5 or 1 mg/kg, i.p.) or the A 2B receptor antagonist alloxazine (1.5 or 3.0 mg/kg, i.p.). 4. Our results suggest that the anticonvulsant and neuroprotective effects of PDTC are mediated, at least in part, via adenosine A1 receptor stimulation.

Original languageEnglish
Pages (from-to)320-326
Number of pages7
JournalClinical and Experimental Pharmacology and Physiology
Volume31
Issue number5-6
DOIs
Publication statusPublished - 2004 May 1
Externally publishedYes

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Kainic Acid
Malondialdehyde
Seizures
Glycyrrhetinic Acid
Adenosine A1 Receptors
Neuroprotective Agents
Anticonvulsants
Adenosine
Sprague Dawley Rats
Hippocampus
Proteins
pyrrolidine dithiocarbamic acid

Keywords

  • Adenosine A receptor
  • Anti-oxidant
  • Anticonvulsant effects
  • Hippocampus
  • Kainic acid
  • Malondialdehyde
  • Neuroprotective effects
  • Protein carbonyl
  • Pyrrolidine dithiocarbamate

ASJC Scopus subject areas

  • Physiology
  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Protection against kainate neurotoxicity by pyrrolidine dithiocarbamate. / Shin, Eun Joo; Jhoo, Jin Hyeong; Kim, Won-Ki; Jhoo, Wang Kee; Lee, Chaeyoung; Jung, Bae Dong; Kim, Hyoung Chun.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 31, No. 5-6, 01.05.2004, p. 320-326.

Research output: Contribution to journalArticle

Shin, Eun Joo ; Jhoo, Jin Hyeong ; Kim, Won-Ki ; Jhoo, Wang Kee ; Lee, Chaeyoung ; Jung, Bae Dong ; Kim, Hyoung Chun. / Protection against kainate neurotoxicity by pyrrolidine dithiocarbamate. In: Clinical and Experimental Pharmacology and Physiology. 2004 ; Vol. 31, No. 5-6. pp. 320-326.
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