Abstract
1. The effect of pyrrolidine dithiocarbamate (PDTC) on kainate (KA)-induced neurotoxicity was examined in Sprague-Dawley rats. 2. At 10 mg/kg, i.p., KA produced seizures accompanied by neuronal loss in the hippocampus and increased levels of malondialdehyde (MDA) and protein carbonyl. 3. Pretreatment with PDTC (100 or 200 mg/kg, p.o., every 12 h x5) blocked KA-induced neurotoxicities (seizures, increases in MDA and protein carbonyl and neuronal losses) in a dose-dependent manner. These effects were counteracted by the adenosine A 1 receptor antagonist 8-cyclopentyl-1,3-dimetliylxanthine (25 or 50 μg/kg, i.p.), but not by the A2A receptor antagonist 1,3,7-trimethyl-8-(3-chlorostyryl)xanthine (0.5 or 1 mg/kg, i.p.) or the A 2B receptor antagonist alloxazine (1.5 or 3.0 mg/kg, i.p.). 4. Our results suggest that the anticonvulsant and neuroprotective effects of PDTC are mediated, at least in part, via adenosine A1 receptor stimulation.
| Original language | English |
|---|---|
| Pages (from-to) | 320-326 |
| Number of pages | 7 |
| Journal | Clinical and Experimental Pharmacology and Physiology |
| Volume | 31 |
| Issue number | 5-6 |
| DOIs | |
| Publication status | Published - 2004 May 1 |
| Externally published | Yes |
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Keywords
- Adenosine A receptor
- Anti-oxidant
- Anticonvulsant effects
- Hippocampus
- Kainic acid
- Malondialdehyde
- Neuroprotective effects
- Protein carbonyl
- Pyrrolidine dithiocarbamate
ASJC Scopus subject areas
- Physiology
- Pharmacology (medical)
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Protection against kainate neurotoxicity by pyrrolidine dithiocarbamate. / Shin, Eun Joo; Jhoo, Jin Hyeong; Kim, Won-Ki; Jhoo, Wang Kee; Lee, Chaeyoung; Jung, Bae Dong; Kim, Hyoung Chun.
In: Clinical and Experimental Pharmacology and Physiology, Vol. 31, No. 5-6, 01.05.2004, p. 320-326.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protection against kainate neurotoxicity by pyrrolidine dithiocarbamate
AU - Shin, Eun Joo
AU - Jhoo, Jin Hyeong
AU - Kim, Won-Ki
AU - Jhoo, Wang Kee
AU - Lee, Chaeyoung
AU - Jung, Bae Dong
AU - Kim, Hyoung Chun
PY - 2004/5/1
Y1 - 2004/5/1
N2 - 1. The effect of pyrrolidine dithiocarbamate (PDTC) on kainate (KA)-induced neurotoxicity was examined in Sprague-Dawley rats. 2. At 10 mg/kg, i.p., KA produced seizures accompanied by neuronal loss in the hippocampus and increased levels of malondialdehyde (MDA) and protein carbonyl. 3. Pretreatment with PDTC (100 or 200 mg/kg, p.o., every 12 h x5) blocked KA-induced neurotoxicities (seizures, increases in MDA and protein carbonyl and neuronal losses) in a dose-dependent manner. These effects were counteracted by the adenosine A 1 receptor antagonist 8-cyclopentyl-1,3-dimetliylxanthine (25 or 50 μg/kg, i.p.), but not by the A2A receptor antagonist 1,3,7-trimethyl-8-(3-chlorostyryl)xanthine (0.5 or 1 mg/kg, i.p.) or the A 2B receptor antagonist alloxazine (1.5 or 3.0 mg/kg, i.p.). 4. Our results suggest that the anticonvulsant and neuroprotective effects of PDTC are mediated, at least in part, via adenosine A1 receptor stimulation.
AB - 1. The effect of pyrrolidine dithiocarbamate (PDTC) on kainate (KA)-induced neurotoxicity was examined in Sprague-Dawley rats. 2. At 10 mg/kg, i.p., KA produced seizures accompanied by neuronal loss in the hippocampus and increased levels of malondialdehyde (MDA) and protein carbonyl. 3. Pretreatment with PDTC (100 or 200 mg/kg, p.o., every 12 h x5) blocked KA-induced neurotoxicities (seizures, increases in MDA and protein carbonyl and neuronal losses) in a dose-dependent manner. These effects were counteracted by the adenosine A 1 receptor antagonist 8-cyclopentyl-1,3-dimetliylxanthine (25 or 50 μg/kg, i.p.), but not by the A2A receptor antagonist 1,3,7-trimethyl-8-(3-chlorostyryl)xanthine (0.5 or 1 mg/kg, i.p.) or the A 2B receptor antagonist alloxazine (1.5 or 3.0 mg/kg, i.p.). 4. Our results suggest that the anticonvulsant and neuroprotective effects of PDTC are mediated, at least in part, via adenosine A1 receptor stimulation.
KW - Adenosine A receptor
KW - Anti-oxidant
KW - Anticonvulsant effects
KW - Hippocampus
KW - Kainic acid
KW - Malondialdehyde
KW - Neuroprotective effects
KW - Protein carbonyl
KW - Pyrrolidine dithiocarbamate
UR - http://www.scopus.com/inward/record.url?scp=3042624969&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042624969&partnerID=8YFLogxK
U2 - 10.1111/j.1440-1681.2004.03990.x
DO - 10.1111/j.1440-1681.2004.03990.x
M3 - Article
VL - 31
SP - 320
EP - 326
JO - Clinical and Experimental Pharmacology and Physiology
JF - Clinical and Experimental Pharmacology and Physiology
SN - 0305-1870
IS - 5-6
ER -