Protective effect of β-caryophyllene, a natural bicyclic sesquiterpene, against cerebral ischemic injury

Hyun Joo Chang, Ji Myung Kim, Jae Chul Lee, Won-Ki Kim, Hyang Sook Chun

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

β-Caryophyllene (trans-4,11,11-trimethyl-8-methylenebicyclo[7,2,0] undec-4-ene), found in various plants, is a natural bicyclic sesquiterpene with a low toxicity. Here, we show that a single intraperitoneal injection of β-caryophyllene (10 mg/kg) significantly reduced the cortical infarct volume by 67% when given immediately before middle cerebral artery occlusion (MCAO). Neurological deficits caused by MCAO were also significantly decreased by β-caryophyllene. β-Caryophyllene treatment of cortical cells exposed to oxygen-glucose deprivation revealed a significant protection in a dose-dependent manner. However, β-caryophyllene neither suppressed N-methyl-d-aspartate excitotoxicity in cultured cortical cells nor markedly decreased the oxidative stress measured in the cellular or acellular systems. By contrast, treatments with β-caryophyllene dose-dependently inhibited mRNA expression of inducible nitric oxide synthetase, interleukin (IL)-1β, IL-6, and cyclooxygenase 2 in C6 microglial cells, and decreased the level of nitric oxide and prostaglandin E2 at a 100 μM concentration. All of these findings suggest that β-caryophyllene has a potent neuroprotective activity, and its neuroprotection may be partly related to the modulation of inflammatory mediators.

Original languageEnglish
Pages (from-to)471-480
Number of pages10
JournalJournal of Medicinal Food
Volume16
Issue number6
DOIs
Publication statusPublished - 2013 Jun 1

Fingerprint

Sesquiterpenes
Wounds and Injuries
Middle Cerebral Artery Infarction
Cyclooxygenase 2
caryophyllene
Intraperitoneal Injections
Interleukin-1
Dinoprostone
Aspartic Acid
Nitric Oxide Synthase
Interleukin-2
Cultured Cells
Interleukin-6
Nitric Oxide
Oxidative Stress
Oxygen
Glucose
Messenger RNA
Therapeutics

Keywords

  • β-caryophyllene
  • Infarct volume
  • Inflammatory mediators
  • Middle cerebral artery occlusion
  • Neuroprotective

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Protective effect of β-caryophyllene, a natural bicyclic sesquiterpene, against cerebral ischemic injury. / Chang, Hyun Joo; Kim, Ji Myung; Lee, Jae Chul; Kim, Won-Ki; Chun, Hyang Sook.

In: Journal of Medicinal Food, Vol. 16, No. 6, 01.06.2013, p. 471-480.

Research output: Contribution to journalArticle

Chang, Hyun Joo ; Kim, Ji Myung ; Lee, Jae Chul ; Kim, Won-Ki ; Chun, Hyang Sook. / Protective effect of β-caryophyllene, a natural bicyclic sesquiterpene, against cerebral ischemic injury. In: Journal of Medicinal Food. 2013 ; Vol. 16, No. 6. pp. 471-480.
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