Protective effect of 70% ethanolic extract of Lindera obtusiloba Blume on tert-butyl hydroperoxide-induced oxidative hepatotoxicity in rats

Chung Oui Hong, Chae Hong Rhee, Nam Hee Won, Hee Don Choi, Kwang Won Lee

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13 Citations (Scopus)


Lindera obtusiloba Blume, a native plant of East Asia, has traditionally been used as a folk medicine for liver disease. We studied the in vitro antioxidant and in vivo hepatoprotective activities of a 70% ethanolic extract of L. obtusiloba (LOE) containing 62.9% quercitrin and 22.0% afzelin. LOE prevented tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in HepG2 cells. Along with its high antioxidant potency in vitro, our animal study confirmed that pretreatment with LOE (500 or 2000. mg/kg) for 7. days prior to a single dose of t-BHP (i.p.: 0.5. mmol/kg) significantly lowered the serum levels of alanine and aspartate aminotransferases. In addition, glutathione levels were increased in the liver, and lipid peroxidation levels were decreased in a dose-dependent manner. The histopathological examinations of rat livers showed that LOE significantly reduced the incidence of liver lesions induced by t-BHP. Therefore, we concluded that LOE has merit as a potent candidate to protect the liver against oxidative damage.

Original languageEnglish
Pages (from-to)214-220
Number of pages7
JournalFood and Chemical Toxicology
Publication statusPublished - 2013 Mar 1



  • Afzelin
  • Hepatotoxicity
  • Lindera obtusiloba
  • Oxidative stress
  • Quercitrin
  • Tert-Butyl hydroperoxide

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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