Protective effect of klotho protein against cisplatin ototoxicity in an auditory cell line

Se Jin Park, So Hyun Park, Ji Won Chang, June Choi, Hak Hyun Jung, Gi Jung Im

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: Klotho protein is involved in insulin-signalling and ageing. Klotho mutation causes premature ageing and significantly shortens the lifespan. The anti-neoplastic drug cisplatin promotes ototoxicity at higher doses by inducing apoptosis. This study aimed to clarify the effect of klotho expression on cisplatin ototoxicity, using an auditory cell line. Materials and methods: Expressions of klotho messenger RNA and protein were analysed by reverse-transcription polymerase chain reaction and western blotting. Auditory cells (HEI-OC1 line) were pretreated with 2 nM klotho protein for 2 hours; 15 M cisplatin was then applied. After 48 hours incubation, assessment of cell viability (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay), apoptosis (via Hoechst 33258 staining) and reactive oxygen species was performed. Results: Klotho protein expression increased in cisplatin-treated auditory cells. Cells treated with both klotho protein and cisplatin showed a viability of 67.7 per cent, versus 59.4 per cent in cisplatin-treated cells. Klotho significantly attenuated the cisplatin-induced increase in reactive oxygen species, and increased the viability of cells with cisplatin-induced cytotoxicity. Conclusion: Klotho protein is protective against cisplatin-induced auditory cell cytotoxicity; inhibition of reactive oxygen species may be the main mechanism.

Original languageEnglish
Pages (from-to)1003-1009
Number of pages7
JournalJournal of Laryngology and Otology
Volume126
Issue number10
DOIs
Publication statusPublished - 2012 Oct

Keywords

  • Auditory Hair Cell
  • Cisplatin
  • Klotho Protein
  • Mouse
  • Reactive Oxygen Species

ASJC Scopus subject areas

  • Otorhinolaryngology

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