Protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells

Se Hee Lee, Hyung Sub Kim, Yun Suk An, Jiwon Chang, June Choi, Gi Jung Im

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objectives: Cisplatin is an effective chemotherapeutic drug, but it generates reactive oxygen species (ROS) that induce severe adverse effects such as ototoxicity. Resveratrol reportedly prevents oxidative stress-induced cell death. Thus, we hypothesized that the anti-oxidative effect of resveratrol could protect against cisplatin-induced ototoxicity. The present study examined the protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells. Methods: HEI-OC1 cells were pretreated with resveratrol at 1. μM for 24. h and then exposed to 15. μM cisplatin for 48. h. Resulting cytotoxicity was measured by the MTT method, and intracellular ROS was measured using flow cytometry. Protective effect of resveratrol was compared with other anti-oxidants. Results: Pretreatment with resveratrol 1. μM protected HEI-OC1 auditory cells against cisplatin-induced cytotoxicity and significantly reduced a cisplatin-induced increase in ROS. Resveratrol provided significant protection against 15. μM cisplatin applied for 48. h (50.8% cell viability in the cisplatin group vs. 57.6% in the cisplatin-plus-resveratrol group), and there was a 9% decrease in cisplatin-induced ROS associated with resveratrol. Conclusions: This is the study investigating the protective effects of resveratrol against cisplatin-induced ototoxicity in an auditory cell line. Resveratrol significantly reduced a cisplatin-induced increase in ROS and thereby inhibited cisplatin-induced cytotoxicity.

Original languageEnglish
Pages (from-to)58-62
Number of pages5
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume79
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

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Cisplatin
Reactive Oxygen Species
resveratrol
Oxidants
Cell Survival
Flow Cytometry
Oxidative Stress
Cell Death
Cell Line

Keywords

  • Cisplatin
  • Ototoxicity
  • Reactive oxygen species
  • Resveratrol

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Otorhinolaryngology

Cite this

Protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells. / Lee, Se Hee; Kim, Hyung Sub; An, Yun Suk; Chang, Jiwon; Choi, June; Im, Gi Jung.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 79, No. 1, 01.01.2015, p. 58-62.

Research output: Contribution to journalArticle

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abstract = "Objectives: Cisplatin is an effective chemotherapeutic drug, but it generates reactive oxygen species (ROS) that induce severe adverse effects such as ototoxicity. Resveratrol reportedly prevents oxidative stress-induced cell death. Thus, we hypothesized that the anti-oxidative effect of resveratrol could protect against cisplatin-induced ototoxicity. The present study examined the protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells. Methods: HEI-OC1 cells were pretreated with resveratrol at 1. μM for 24. h and then exposed to 15. μM cisplatin for 48. h. Resulting cytotoxicity was measured by the MTT method, and intracellular ROS was measured using flow cytometry. Protective effect of resveratrol was compared with other anti-oxidants. Results: Pretreatment with resveratrol 1. μM protected HEI-OC1 auditory cells against cisplatin-induced cytotoxicity and significantly reduced a cisplatin-induced increase in ROS. Resveratrol provided significant protection against 15. μM cisplatin applied for 48. h (50.8{\%} cell viability in the cisplatin group vs. 57.6{\%} in the cisplatin-plus-resveratrol group), and there was a 9{\%} decrease in cisplatin-induced ROS associated with resveratrol. Conclusions: This is the study investigating the protective effects of resveratrol against cisplatin-induced ototoxicity in an auditory cell line. Resveratrol significantly reduced a cisplatin-induced increase in ROS and thereby inhibited cisplatin-induced cytotoxicity.",
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AU - Choi, June

AU - Im, Gi Jung

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N2 - Objectives: Cisplatin is an effective chemotherapeutic drug, but it generates reactive oxygen species (ROS) that induce severe adverse effects such as ototoxicity. Resveratrol reportedly prevents oxidative stress-induced cell death. Thus, we hypothesized that the anti-oxidative effect of resveratrol could protect against cisplatin-induced ototoxicity. The present study examined the protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells. Methods: HEI-OC1 cells were pretreated with resveratrol at 1. μM for 24. h and then exposed to 15. μM cisplatin for 48. h. Resulting cytotoxicity was measured by the MTT method, and intracellular ROS was measured using flow cytometry. Protective effect of resveratrol was compared with other anti-oxidants. Results: Pretreatment with resveratrol 1. μM protected HEI-OC1 auditory cells against cisplatin-induced cytotoxicity and significantly reduced a cisplatin-induced increase in ROS. Resveratrol provided significant protection against 15. μM cisplatin applied for 48. h (50.8% cell viability in the cisplatin group vs. 57.6% in the cisplatin-plus-resveratrol group), and there was a 9% decrease in cisplatin-induced ROS associated with resveratrol. Conclusions: This is the study investigating the protective effects of resveratrol against cisplatin-induced ototoxicity in an auditory cell line. Resveratrol significantly reduced a cisplatin-induced increase in ROS and thereby inhibited cisplatin-induced cytotoxicity.

AB - Objectives: Cisplatin is an effective chemotherapeutic drug, but it generates reactive oxygen species (ROS) that induce severe adverse effects such as ototoxicity. Resveratrol reportedly prevents oxidative stress-induced cell death. Thus, we hypothesized that the anti-oxidative effect of resveratrol could protect against cisplatin-induced ototoxicity. The present study examined the protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells. Methods: HEI-OC1 cells were pretreated with resveratrol at 1. μM for 24. h and then exposed to 15. μM cisplatin for 48. h. Resulting cytotoxicity was measured by the MTT method, and intracellular ROS was measured using flow cytometry. Protective effect of resveratrol was compared with other anti-oxidants. Results: Pretreatment with resveratrol 1. μM protected HEI-OC1 auditory cells against cisplatin-induced cytotoxicity and significantly reduced a cisplatin-induced increase in ROS. Resveratrol provided significant protection against 15. μM cisplatin applied for 48. h (50.8% cell viability in the cisplatin group vs. 57.6% in the cisplatin-plus-resveratrol group), and there was a 9% decrease in cisplatin-induced ROS associated with resveratrol. Conclusions: This is the study investigating the protective effects of resveratrol against cisplatin-induced ototoxicity in an auditory cell line. Resveratrol significantly reduced a cisplatin-induced increase in ROS and thereby inhibited cisplatin-induced cytotoxicity.

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