Protective effects of adipose-derived stem cells against UVB-induced skin pigmentation

Byung Joon Jeon, Deok-Woo Kim, Min Sook Kim, Seung Ha Park, Eun-Sang Dhong, Eul Sik Yoon, Byung-Il Lee, Na Hyun Hwang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Hyperpigmentation, mainly following UV-irradiation, can cause major cosmetic concerns. Human adipose tissue-derived stem cells (ASCs) have been reported to serve as whitening agents through a paracrine effect. However, there have been few reports on the direct effects of ASCs on skin pigmentation following UVB-irradiation. Methods: To evaluate the effect of ASCs on UVB-irradiated mouse skin, UVB-irradiation alone was applied to one side of the backs of mice (melanin-processing hairless mouse, HRM-2) as a control, and UVB-irradiation plus injection of ASCs was applied to the contralateral side. Skin pigmentation and histology were evaluated and the number of DOPA-positive melanocytes in the mouse skin was counted. The absolute value of ΔL* via a colorimeter was measured to evaluate the degree of skin pigmentation. The effects of ASCs on the melanogenic activities of mouse skin were examined by measuring the tyrosinase activity and the melanin contents in the epidermis of the mouse skin. Results: Skin pigmentation was suppressed in the ASC-injected side. Moreover, the change in skin thickness following UVB irradiation was reduced in the ASC-injected side. The number of DOPA-positive melanocytes in the ASC-injected side (139 ± 18 cells/mm2) was significantly lower than that in the control side (239 ± 48 cells/mm2). The tyrosinase activity (67.4 ± 9.8% of that of the control side) and melanin content (63.4 ± 5.7% of that of the control side) of the ASC-injected side were also significantly reduced. Conclusions: Collectively, these results suggest that ASCs injected subcutaneously into the backs of mice can attenuate tanning following UVB-irradiation, through suppression of tyrosinase activity.

Original languageEnglish
Pages (from-to)336-342
Number of pages7
JournalJournal of Plastic Surgery and Hand Surgery
Volume50
Issue number6
DOIs
Publication statusPublished - 2016 Nov 1

Fingerprint

Skin Pigmentation
Adipose Tissue
Stem Cells
Monophenol Monooxygenase
Melanins
Skin
Melanocytes
Bleaching Agents
Tanning
Hairless Mouse
Hyperpigmentation
Epidermis
Cosmetics
Histology

Keywords

  • Adipose-derived stem cells
  • pigmentation
  • UVB

ASJC Scopus subject areas

  • Surgery

Cite this

Protective effects of adipose-derived stem cells against UVB-induced skin pigmentation. / Jeon, Byung Joon; Kim, Deok-Woo; Kim, Min Sook; Park, Seung Ha; Dhong, Eun-Sang; Yoon, Eul Sik; Lee, Byung-Il; Hwang, Na Hyun.

In: Journal of Plastic Surgery and Hand Surgery, Vol. 50, No. 6, 01.11.2016, p. 336-342.

Research output: Contribution to journalArticle

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abstract = "Background: Hyperpigmentation, mainly following UV-irradiation, can cause major cosmetic concerns. Human adipose tissue-derived stem cells (ASCs) have been reported to serve as whitening agents through a paracrine effect. However, there have been few reports on the direct effects of ASCs on skin pigmentation following UVB-irradiation. Methods: To evaluate the effect of ASCs on UVB-irradiated mouse skin, UVB-irradiation alone was applied to one side of the backs of mice (melanin-processing hairless mouse, HRM-2) as a control, and UVB-irradiation plus injection of ASCs was applied to the contralateral side. Skin pigmentation and histology were evaluated and the number of DOPA-positive melanocytes in the mouse skin was counted. The absolute value of ΔL* via a colorimeter was measured to evaluate the degree of skin pigmentation. The effects of ASCs on the melanogenic activities of mouse skin were examined by measuring the tyrosinase activity and the melanin contents in the epidermis of the mouse skin. Results: Skin pigmentation was suppressed in the ASC-injected side. Moreover, the change in skin thickness following UVB irradiation was reduced in the ASC-injected side. The number of DOPA-positive melanocytes in the ASC-injected side (139 ± 18 cells/mm2) was significantly lower than that in the control side (239 ± 48 cells/mm2). The tyrosinase activity (67.4 ± 9.8{\%} of that of the control side) and melanin content (63.4 ± 5.7{\%} of that of the control side) of the ASC-injected side were also significantly reduced. Conclusions: Collectively, these results suggest that ASCs injected subcutaneously into the backs of mice can attenuate tanning following UVB-irradiation, through suppression of tyrosinase activity.",
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AU - Dhong, Eun-Sang

AU - Yoon, Eul Sik

AU - Lee, Byung-Il

AU - Hwang, Na Hyun

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