Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish

Moo Kyun Park, Gi Jung Im, Jiwon Chang, Sungwon Chae, Jun Yoo, Won gue Han, Gyu Ho Hwang, Jong Yoon Jung, Jungim Choi, Hak Hyun Jung, Ah Young Chung, Hae Chul Park, June Choi

Research output: Contribution to journalArticle

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Abstract

Objective: Caffeic acid phenethyl ester (CAPE) is known to reduce the generation of oxygen-derived free radicals, which is a major mechanism of aminoglycoside-induced ototoxicity. The objective of the present study was to evaluate the effects of CAPE on neomycin-induced ototoxicity in zebrafish (Brn3c: EGFP). Methods: Five-day post-fertilization zebrafish larvae (n= 10) were exposed to 125. μM neomycin and one of the following CAPE concentrations for 1. h: 50, 100, 250, 500, or 1000. μM. Ultrastructural changes were evaluated using scanning electron microscopy (SEM). The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay and 2-[4-(dimethylamino)styryl]- N-ethylpyridiniumiodide (DASPEI) assay were performed for evaluation of apoptosis and mitochondrial damage. Results: CAPE decreased neomycin-induced hair cell loss in the neuromasts (500. μM CAPE: 12.7 ± 1.1 cells, 125. μM neomycin only: 6.3 ± 1.1 cells; n= 10, P < 0.05). In the ultrastructural analysis, structures of mitochondria and hair cells were preserved when exposed to 125. μM neomycin and 500. μM CAPE. CAPE decreased apoptosis and mitochondrial damage. Conclusion: In the present study, CAPE attenuated neomycin-induced hair cell damage in zebrafish. The results of the current study suggest that neomycin induces apoptosis, and the apoptotic cell death can be prevented by treatment with CAPE in zebrafish.

Original languageEnglish
Pages (from-to)1311-1315
Number of pages5
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume78
Issue number8
DOIs
Publication statusPublished - 2014 Jan 1

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Neomycin
Zebrafish
Apoptosis
caffeic acid phenethyl ester
DNA Nucleotidylexotransferase
Alopecia
Aminoglycosides
Biotin
Fertilization
Electron Scanning Microscopy
Free Radicals
Larva
Mitochondria
Cell Death
Oxygen

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Pediatrics, Perinatology, and Child Health

Cite this

Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish. / Park, Moo Kyun; Im, Gi Jung; Chang, Jiwon; Chae, Sungwon; Yoo, Jun; Han, Won gue; Hwang, Gyu Ho; Jung, Jong Yoon; Choi, Jungim; Jung, Hak Hyun; Chung, Ah Young; Park, Hae Chul; Choi, June.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 78, No. 8, 01.01.2014, p. 1311-1315.

Research output: Contribution to journalArticle

Park, Moo Kyun ; Im, Gi Jung ; Chang, Jiwon ; Chae, Sungwon ; Yoo, Jun ; Han, Won gue ; Hwang, Gyu Ho ; Jung, Jong Yoon ; Choi, Jungim ; Jung, Hak Hyun ; Chung, Ah Young ; Park, Hae Chul ; Choi, June. / Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish. In: International Journal of Pediatric Otorhinolaryngology. 2014 ; Vol. 78, No. 8. pp. 1311-1315.
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abstract = "Objective: Caffeic acid phenethyl ester (CAPE) is known to reduce the generation of oxygen-derived free radicals, which is a major mechanism of aminoglycoside-induced ototoxicity. The objective of the present study was to evaluate the effects of CAPE on neomycin-induced ototoxicity in zebrafish (Brn3c: EGFP). Methods: Five-day post-fertilization zebrafish larvae (n= 10) were exposed to 125. μM neomycin and one of the following CAPE concentrations for 1. h: 50, 100, 250, 500, or 1000. μM. Ultrastructural changes were evaluated using scanning electron microscopy (SEM). The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay and 2-[4-(dimethylamino)styryl]- N-ethylpyridiniumiodide (DASPEI) assay were performed for evaluation of apoptosis and mitochondrial damage. Results: CAPE decreased neomycin-induced hair cell loss in the neuromasts (500. μM CAPE: 12.7 ± 1.1 cells, 125. μM neomycin only: 6.3 ± 1.1 cells; n= 10, P < 0.05). In the ultrastructural analysis, structures of mitochondria and hair cells were preserved when exposed to 125. μM neomycin and 500. μM CAPE. CAPE decreased apoptosis and mitochondrial damage. Conclusion: In the present study, CAPE attenuated neomycin-induced hair cell damage in zebrafish. The results of the current study suggest that neomycin induces apoptosis, and the apoptotic cell death can be prevented by treatment with CAPE in zebrafish.",
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T1 - Protective effects of caffeic acid phenethyl ester (CAPE) against neomycin-induced hair cell damage in zebrafish

AU - Park, Moo Kyun

AU - Im, Gi Jung

AU - Chang, Jiwon

AU - Chae, Sungwon

AU - Yoo, Jun

AU - Han, Won gue

AU - Hwang, Gyu Ho

AU - Jung, Jong Yoon

AU - Choi, Jungim

AU - Jung, Hak Hyun

AU - Chung, Ah Young

AU - Park, Hae Chul

AU - Choi, June

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Objective: Caffeic acid phenethyl ester (CAPE) is known to reduce the generation of oxygen-derived free radicals, which is a major mechanism of aminoglycoside-induced ototoxicity. The objective of the present study was to evaluate the effects of CAPE on neomycin-induced ototoxicity in zebrafish (Brn3c: EGFP). Methods: Five-day post-fertilization zebrafish larvae (n= 10) were exposed to 125. μM neomycin and one of the following CAPE concentrations for 1. h: 50, 100, 250, 500, or 1000. μM. Ultrastructural changes were evaluated using scanning electron microscopy (SEM). The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay and 2-[4-(dimethylamino)styryl]- N-ethylpyridiniumiodide (DASPEI) assay were performed for evaluation of apoptosis and mitochondrial damage. Results: CAPE decreased neomycin-induced hair cell loss in the neuromasts (500. μM CAPE: 12.7 ± 1.1 cells, 125. μM neomycin only: 6.3 ± 1.1 cells; n= 10, P < 0.05). In the ultrastructural analysis, structures of mitochondria and hair cells were preserved when exposed to 125. μM neomycin and 500. μM CAPE. CAPE decreased apoptosis and mitochondrial damage. Conclusion: In the present study, CAPE attenuated neomycin-induced hair cell damage in zebrafish. The results of the current study suggest that neomycin induces apoptosis, and the apoptotic cell death can be prevented by treatment with CAPE in zebrafish.

AB - Objective: Caffeic acid phenethyl ester (CAPE) is known to reduce the generation of oxygen-derived free radicals, which is a major mechanism of aminoglycoside-induced ototoxicity. The objective of the present study was to evaluate the effects of CAPE on neomycin-induced ototoxicity in zebrafish (Brn3c: EGFP). Methods: Five-day post-fertilization zebrafish larvae (n= 10) were exposed to 125. μM neomycin and one of the following CAPE concentrations for 1. h: 50, 100, 250, 500, or 1000. μM. Ultrastructural changes were evaluated using scanning electron microscopy (SEM). The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay and 2-[4-(dimethylamino)styryl]- N-ethylpyridiniumiodide (DASPEI) assay were performed for evaluation of apoptosis and mitochondrial damage. Results: CAPE decreased neomycin-induced hair cell loss in the neuromasts (500. μM CAPE: 12.7 ± 1.1 cells, 125. μM neomycin only: 6.3 ± 1.1 cells; n= 10, P < 0.05). In the ultrastructural analysis, structures of mitochondria and hair cells were preserved when exposed to 125. μM neomycin and 500. μM CAPE. CAPE decreased apoptosis and mitochondrial damage. Conclusion: In the present study, CAPE attenuated neomycin-induced hair cell damage in zebrafish. The results of the current study suggest that neomycin induces apoptosis, and the apoptotic cell death can be prevented by treatment with CAPE in zebrafish.

KW - Caffeic acid phenethyl ester

KW - Neomycin

KW - Ototoxicity

KW - Zebrafish

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