Protective effects of chebulic acid from Terminalia chebula Retz. against t-BHP-induced oxidative stress by modulations of Nrf2 and its related enzymes in HepG2 cells

Hye Lim Jung, Sung Yong Yang, Min Cheol Pyo, Chung Oui Hong, Mi Hyun Nam, Jin Won Lee, Kwang Won Lee

Research output: Contribution to journalArticle

Abstract

Although chebulic acid isolated from Terminalia chebular has diverse biological effects, its effects on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of downstream genes have not been elucidated. The purpose of this research is to investigate the hepatoprotective mechanism of chebulic acid against oxidative stress produced by tert-butyl hydroperoxide (t-BHP) in liver cells. The treatment with chebulic acid attenuated cell death in t-BHP-induced HepG2 liver cells and increased intracellular glutathione content, upregulated the activity of heme oxygenase-1, and also increased the translocation of Nrf2 into the nucleus and Nrf2 target gene expression in a dose-dependent manner. The exposure of chebulic acid activated the phosphorylation of mitogen-activated protein kinases. The overall result is that chebulic acid has cytoprotective effect on t-BHP-induced hepatotoxicity in HepG2 cells through Nrf2-mediated antioxidant enzymes.

Original languageEnglish
Pages (from-to)555-562
Number of pages8
JournalFood Science and Biotechnology
Volume28
Issue number2
DOIs
Publication statusPublished - 2019 Apr 5

Keywords

  • Chebulic acid
  • Heme oxygenase-1
  • Mitogen-activated protein kinases
  • Nuclear factor erythroid 2-related factor 2
  • γ-Glutamate cysteine ligase

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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