Abstract
Background: Chebulic acid (CA) isolated from T. chebula, which has been reported for treating asthma, as a potent anti-oxidant resources. Exposure to ambient urban particulate matter (UPM) considered as a risk for cardiopulmonary vascular dysfunction. To investigate the protective effect of CA against UPM-mediated collapse of the pulmonary alveolar epithelial (PAE) cell (NCI-H441), barrier integrity parameters, and their elements were evaluated in PAE. Methods: CA was acquired from the laboratory previous reports. UPM was obtained from the National Institutes of Standards and Technology, and these were collected in St. Louis, MO, over a 24-month period and used as a standard reference. To confirm the protection of PAE barrier integrity, paracellular permeability and the junctional molecules were estimated with determination of transepithelial electrical resistance, Western Blotting, RT-PCR, and fluorescent staining. Results: UPM aggravated the generation of reactive oxygen species (ROS) in PAE and also decreased mRNA and protein levels of junction molecules and barrier integrity in NCI-H441. However, CA repressed the ROS in PAE, also improved barrier integrity by protecting the junctional parameters in NCI-H411. Conclusions: These data showed that CA resulted in decreased UPM-induced ROS formation, and the protected the integrity of the tight junctions against UPM exposure to PAE barrier.
| Original language | English |
|---|---|
| Article number | 373 |
| Journal | BMC Complementary and Alternative Medicine |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2017 Jul 19 |
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Keywords
- Alveolar barrier dysfunction
- Chebulic acid
- Inflammation
- Pulmonary alveolus
- Urban particulate matter
ASJC Scopus subject areas
- Complementary and alternative medicine
Cite this
Protective effects of chebulic acid on alveolar epithelial damage induced by urban particulate matter. / Lee, Kyung Won; Nam, Mi Hyun; Lee, Hee Ra; Hong, Chung Oui; Lee, Kwang Won.
In: BMC Complementary and Alternative Medicine, Vol. 17, No. 1, 373, 19.07.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protective effects of chebulic acid on alveolar epithelial damage induced by urban particulate matter
AU - Lee, Kyung Won
AU - Nam, Mi Hyun
AU - Lee, Hee Ra
AU - Hong, Chung Oui
AU - Lee, Kwang Won
PY - 2017/7/19
Y1 - 2017/7/19
N2 - Background: Chebulic acid (CA) isolated from T. chebula, which has been reported for treating asthma, as a potent anti-oxidant resources. Exposure to ambient urban particulate matter (UPM) considered as a risk for cardiopulmonary vascular dysfunction. To investigate the protective effect of CA against UPM-mediated collapse of the pulmonary alveolar epithelial (PAE) cell (NCI-H441), barrier integrity parameters, and their elements were evaluated in PAE. Methods: CA was acquired from the laboratory previous reports. UPM was obtained from the National Institutes of Standards and Technology, and these were collected in St. Louis, MO, over a 24-month period and used as a standard reference. To confirm the protection of PAE barrier integrity, paracellular permeability and the junctional molecules were estimated with determination of transepithelial electrical resistance, Western Blotting, RT-PCR, and fluorescent staining. Results: UPM aggravated the generation of reactive oxygen species (ROS) in PAE and also decreased mRNA and protein levels of junction molecules and barrier integrity in NCI-H441. However, CA repressed the ROS in PAE, also improved barrier integrity by protecting the junctional parameters in NCI-H411. Conclusions: These data showed that CA resulted in decreased UPM-induced ROS formation, and the protected the integrity of the tight junctions against UPM exposure to PAE barrier.
AB - Background: Chebulic acid (CA) isolated from T. chebula, which has been reported for treating asthma, as a potent anti-oxidant resources. Exposure to ambient urban particulate matter (UPM) considered as a risk for cardiopulmonary vascular dysfunction. To investigate the protective effect of CA against UPM-mediated collapse of the pulmonary alveolar epithelial (PAE) cell (NCI-H441), barrier integrity parameters, and their elements were evaluated in PAE. Methods: CA was acquired from the laboratory previous reports. UPM was obtained from the National Institutes of Standards and Technology, and these were collected in St. Louis, MO, over a 24-month period and used as a standard reference. To confirm the protection of PAE barrier integrity, paracellular permeability and the junctional molecules were estimated with determination of transepithelial electrical resistance, Western Blotting, RT-PCR, and fluorescent staining. Results: UPM aggravated the generation of reactive oxygen species (ROS) in PAE and also decreased mRNA and protein levels of junction molecules and barrier integrity in NCI-H441. However, CA repressed the ROS in PAE, also improved barrier integrity by protecting the junctional parameters in NCI-H411. Conclusions: These data showed that CA resulted in decreased UPM-induced ROS formation, and the protected the integrity of the tight junctions against UPM exposure to PAE barrier.
KW - Alveolar barrier dysfunction
KW - Chebulic acid
KW - Inflammation
KW - Pulmonary alveolus
KW - Urban particulate matter
UR - http://www.scopus.com/inward/record.url?scp=85025067617&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85025067617&partnerID=8YFLogxK
U2 - 10.1186/s12906-017-1870-5
DO - 10.1186/s12906-017-1870-5
M3 - Article
VL - 17
JO - BMC Complementary and Alternative Medicine
JF - BMC Complementary and Alternative Medicine
SN - 1472-6882
IS - 1
M1 - 373
ER -