Protective immunity against challenge infection with Trichinella spiralis in the rat

Fu Shi Quan, Jun Sang Bae, Jeong Beom Lee, Young Ki Min, Hun Mo Yang, Kyung Whan Joo, Sung-Weon Cho, Joon Sang Lee, Toshiyuki Nakayama, Ichiro Sekine

Research output: Contribution to journalArticle

Abstract

Protective immunity and immune response were studied in rats after primarily infection with Trichinella spiralis. The parameters measured include serum antibody isotype responses, mesenteric lymph node (MLN) cell and spleen cell proliferation responses in vitro, and the levels of interferon (IFN)-γ, interleukin (IL)-2, IL-4 and IL-10 as markers of the T-helper (Th) subset. Protective immunity was assessed by the degree of expulsion of adult worms from the rat intestine. Protective immunity against adult worms after challenge infection was 99.80%. Although IFN-γ, IL-2, IL-4 and IL-10 in both the MLNs and spleen were detected, IFN-γ and IL-2 levels were higher in the spleen than in the MLNs, and IL-4 and an increased amount of IL-10 was released in the MLNs as compared to the spleen. The levels of the specific immunoglobulins (Ig) G, IgM, IgG1 and IgG2a on week 6 after primary infection, and on day 7 after challenge infection, were higher as compared to levels in uninfected rats that only received a challenge infection (P < 0.001), whereas the antibody level of IgG1 was significantly elevated from that of IgG2a (P < 0.001). These results demonstrate that Th1 type responses predominated. In addition, as Th2 type cytokines were also produced, it is proposed that the protective immunity by primary infection was also related to the Th1 type responses.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalActa Medica Nagasakiensia
Volume52
Issue number4
Publication statusPublished - 2007 Dec 1

Fingerprint

Trichinella spiralis
Immunity
Spleen
Infection
Interleukin-4
Interleukin-10
Interferons
Interleukin-2
Immunoglobulin G
Intestines
Antibody Formation
Immunoglobulin M
Lymph Nodes
Cell Proliferation
Cytokines
Antibodies
Serum

Keywords

  • Antibody isotype response
  • Excretory-secretory antigen
  • Infection
  • T helper type cytokine
  • Trichinella spiralis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Quan, F. S., Bae, J. S., Lee, J. B., Min, Y. K., Yang, H. M., Joo, K. W., ... Sekine, I. (2007). Protective immunity against challenge infection with Trichinella spiralis in the rat. Acta Medica Nagasakiensia, 52(4), 101-106.

Protective immunity against challenge infection with Trichinella spiralis in the rat. / Quan, Fu Shi; Bae, Jun Sang; Lee, Jeong Beom; Min, Young Ki; Yang, Hun Mo; Joo, Kyung Whan; Cho, Sung-Weon; Lee, Joon Sang; Nakayama, Toshiyuki; Sekine, Ichiro.

In: Acta Medica Nagasakiensia, Vol. 52, No. 4, 01.12.2007, p. 101-106.

Research output: Contribution to journalArticle

Quan, FS, Bae, JS, Lee, JB, Min, YK, Yang, HM, Joo, KW, Cho, S-W, Lee, JS, Nakayama, T & Sekine, I 2007, 'Protective immunity against challenge infection with Trichinella spiralis in the rat', Acta Medica Nagasakiensia, vol. 52, no. 4, pp. 101-106.
Quan FS, Bae JS, Lee JB, Min YK, Yang HM, Joo KW et al. Protective immunity against challenge infection with Trichinella spiralis in the rat. Acta Medica Nagasakiensia. 2007 Dec 1;52(4):101-106.
Quan, Fu Shi ; Bae, Jun Sang ; Lee, Jeong Beom ; Min, Young Ki ; Yang, Hun Mo ; Joo, Kyung Whan ; Cho, Sung-Weon ; Lee, Joon Sang ; Nakayama, Toshiyuki ; Sekine, Ichiro. / Protective immunity against challenge infection with Trichinella spiralis in the rat. In: Acta Medica Nagasakiensia. 2007 ; Vol. 52, No. 4. pp. 101-106.
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