Abstract
Protective immunity and immune response were studied in rats after primarily infection with Trichinella spiralis. The parameters measured include serum antibody isotype responses, mesenteric lymph node (MLN) cell and spleen cell proliferation responses in vitro, and the levels of interferon (IFN)-γ, interleukin (IL)-2, IL-4 and IL-10 as markers of the T-helper (Th) subset. Protective immunity was assessed by the degree of expulsion of adult worms from the rat intestine. Protective immunity against adult worms after challenge infection was 99.80%. Although IFN-γ, IL-2, IL-4 and IL-10 in both the MLNs and spleen were detected, IFN-γ and IL-2 levels were higher in the spleen than in the MLNs, and IL-4 and an increased amount of IL-10 was released in the MLNs as compared to the spleen. The levels of the specific immunoglobulins (Ig) G, IgM, IgG1 and IgG2a on week 6 after primary infection, and on day 7 after challenge infection, were higher as compared to levels in uninfected rats that only received a challenge infection (P < 0.001), whereas the antibody level of IgG1 was significantly elevated from that of IgG2a (P < 0.001). These results demonstrate that Th1 type responses predominated. In addition, as Th2 type cytokines were also produced, it is proposed that the protective immunity by primary infection was also related to the Th1 type responses.
| Original language | English |
|---|---|
| Pages (from-to) | 101-106 |
| Number of pages | 6 |
| Journal | Acta Medica Nagasakiensia |
| Volume | 52 |
| Issue number | 4 |
| Publication status | Published - 2007 Dec 1 |
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Keywords
- Antibody isotype response
- Excretory-secretory antigen
- Infection
- T helper type cytokine
- Trichinella spiralis
ASJC Scopus subject areas
- Medicine(all)
Cite this
Protective immunity against challenge infection with Trichinella spiralis in the rat. / Quan, Fu Shi; Bae, Jun Sang; Lee, Jeong Beom; Min, Young Ki; Yang, Hun Mo; Joo, Kyung Whan; Cho, Sung-Weon; Lee, Joon Sang; Nakayama, Toshiyuki; Sekine, Ichiro.
In: Acta Medica Nagasakiensia, Vol. 52, No. 4, 01.12.2007, p. 101-106.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protective immunity against challenge infection with Trichinella spiralis in the rat
AU - Quan, Fu Shi
AU - Bae, Jun Sang
AU - Lee, Jeong Beom
AU - Min, Young Ki
AU - Yang, Hun Mo
AU - Joo, Kyung Whan
AU - Cho, Sung-Weon
AU - Lee, Joon Sang
AU - Nakayama, Toshiyuki
AU - Sekine, Ichiro
PY - 2007/12/1
Y1 - 2007/12/1
N2 - Protective immunity and immune response were studied in rats after primarily infection with Trichinella spiralis. The parameters measured include serum antibody isotype responses, mesenteric lymph node (MLN) cell and spleen cell proliferation responses in vitro, and the levels of interferon (IFN)-γ, interleukin (IL)-2, IL-4 and IL-10 as markers of the T-helper (Th) subset. Protective immunity was assessed by the degree of expulsion of adult worms from the rat intestine. Protective immunity against adult worms after challenge infection was 99.80%. Although IFN-γ, IL-2, IL-4 and IL-10 in both the MLNs and spleen were detected, IFN-γ and IL-2 levels were higher in the spleen than in the MLNs, and IL-4 and an increased amount of IL-10 was released in the MLNs as compared to the spleen. The levels of the specific immunoglobulins (Ig) G, IgM, IgG1 and IgG2a on week 6 after primary infection, and on day 7 after challenge infection, were higher as compared to levels in uninfected rats that only received a challenge infection (P < 0.001), whereas the antibody level of IgG1 was significantly elevated from that of IgG2a (P < 0.001). These results demonstrate that Th1 type responses predominated. In addition, as Th2 type cytokines were also produced, it is proposed that the protective immunity by primary infection was also related to the Th1 type responses.
AB - Protective immunity and immune response were studied in rats after primarily infection with Trichinella spiralis. The parameters measured include serum antibody isotype responses, mesenteric lymph node (MLN) cell and spleen cell proliferation responses in vitro, and the levels of interferon (IFN)-γ, interleukin (IL)-2, IL-4 and IL-10 as markers of the T-helper (Th) subset. Protective immunity was assessed by the degree of expulsion of adult worms from the rat intestine. Protective immunity against adult worms after challenge infection was 99.80%. Although IFN-γ, IL-2, IL-4 and IL-10 in both the MLNs and spleen were detected, IFN-γ and IL-2 levels were higher in the spleen than in the MLNs, and IL-4 and an increased amount of IL-10 was released in the MLNs as compared to the spleen. The levels of the specific immunoglobulins (Ig) G, IgM, IgG1 and IgG2a on week 6 after primary infection, and on day 7 after challenge infection, were higher as compared to levels in uninfected rats that only received a challenge infection (P < 0.001), whereas the antibody level of IgG1 was significantly elevated from that of IgG2a (P < 0.001). These results demonstrate that Th1 type responses predominated. In addition, as Th2 type cytokines were also produced, it is proposed that the protective immunity by primary infection was also related to the Th1 type responses.
KW - Antibody isotype response
KW - Excretory-secretory antigen
KW - Infection
KW - T helper type cytokine
KW - Trichinella spiralis
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M3 - Article
VL - 52
SP - 101
EP - 106
JO - Acta Medica Nagasakiensia
JF - Acta Medica Nagasakiensia
SN - 0001-6055
IS - 4
ER -