Protective immunity and antibody response of rats infected with Trichinella spiralis

Fu Shi Quan, Haeng Sook Lee, Myung Sook Chung, Sung-Weon Cho, Kyoung Hwan Joo, Loon Sang Lee, Han Jong Rim

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To study the protective immunity and antibody (IgG, IgG1 and IgG2a) response against adult and larva infection of T. spiralis Korean isolate in rats.

METHODS: Forty-six rats were randomly divided into 7 groups. Group A (A1, A2, 10 rats) was used for the determination of protective efficacy from adult stage infection, group B (B1, B2, 14 rats) was for the protective efficacy from muscle larva stage infection, group C (C1, C2, 17 rats) was for challenge control, and group D (5 rats) served as normal control. Rats in groups A, B and C were infected with 1,000 T.spiralis muscle larvae, and the infected rats were treated with flubendazole (20 mg/kg, 10 d) at day 7 (A1, A2) and at day 30 (B1, B2). Rats in groups A and B were re-infected with 500 T. spiralis muscle larvae at day 10 after treatment. Rats in groups A1 and B1 were killed at day 7 and day 30 to inspect the reduction of adult worms in the intestines. Rats in groups A2 and B2 were killed at day 30 to detect the reduction of muscle larvae in diaphragms. Rats in groups C and D were killed at the same time, and all rats were bled at the same time. Specific anti-Trichinella IgG, IgG1 and IgG2a were detected by ELISA.

RESULTS: Adult stage infection induced protective efficacy by 100% against adult stage and by 99.96% against larva stage. Larval stage infection induced protective efficacy by 99.92% against adult stage and 99.89% against muscle larvae. Anti-muscle stage larval ES Ag (IgG 3.0, IgG1 2.2, IgG2a 0.8) and anti-adult crude Ag antibodies (IgG 1.9, IgG1 0.8, IgG2a 0.3) significantly increased in the muscle larval stage infection compared to normal control (IgG 0.5, IgG1 0.1, IgG2a 0.1) and adult stage infection (IgG 0.5, IgG1 0.09, IgG2a 0.09) (P < 0.01). Higher specific IgG1 antibody (IgG1 2.2) in larva stage infection was shown than specific IgG2a antibody response (IgG2a 0.8)(P < 0.01).

CONCLUSION: Protective immunity against both adult and larva worms has been induced from adult and muscle larva stage infections of T. spiralis.

Original languageEnglish
Pages (from-to)119-123
Number of pages5
JournalZhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Volume26
Issue number2
Publication statusPublished - 2008 Apr 1
Externally publishedYes

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Trichinella spiralis
Antibody Formation
Immunity
Immunoglobulin G
Larva
Muscles
varespladib methyl
Infection
Antibodies
Trichinella

ASJC Scopus subject areas

  • Medicine(all)

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Protective immunity and antibody response of rats infected with Trichinella spiralis. / Quan, Fu Shi; Lee, Haeng Sook; Chung, Myung Sook; Cho, Sung-Weon; Joo, Kyoung Hwan; Lee, Loon Sang; Rim, Han Jong.

In: Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, Vol. 26, No. 2, 01.04.2008, p. 119-123.

Research output: Contribution to journalArticle

Quan, Fu Shi ; Lee, Haeng Sook ; Chung, Myung Sook ; Cho, Sung-Weon ; Joo, Kyoung Hwan ; Lee, Loon Sang ; Rim, Han Jong. / Protective immunity and antibody response of rats infected with Trichinella spiralis. In: Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases. 2008 ; Vol. 26, No. 2. pp. 119-123.
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T1 - Protective immunity and antibody response of rats infected with Trichinella spiralis

AU - Quan, Fu Shi

AU - Lee, Haeng Sook

AU - Chung, Myung Sook

AU - Cho, Sung-Weon

AU - Joo, Kyoung Hwan

AU - Lee, Loon Sang

AU - Rim, Han Jong

PY - 2008/4/1

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N2 - OBJECTIVE: To study the protective immunity and antibody (IgG, IgG1 and IgG2a) response against adult and larva infection of T. spiralis Korean isolate in rats.METHODS: Forty-six rats were randomly divided into 7 groups. Group A (A1, A2, 10 rats) was used for the determination of protective efficacy from adult stage infection, group B (B1, B2, 14 rats) was for the protective efficacy from muscle larva stage infection, group C (C1, C2, 17 rats) was for challenge control, and group D (5 rats) served as normal control. Rats in groups A, B and C were infected with 1,000 T.spiralis muscle larvae, and the infected rats were treated with flubendazole (20 mg/kg, 10 d) at day 7 (A1, A2) and at day 30 (B1, B2). Rats in groups A and B were re-infected with 500 T. spiralis muscle larvae at day 10 after treatment. Rats in groups A1 and B1 were killed at day 7 and day 30 to inspect the reduction of adult worms in the intestines. Rats in groups A2 and B2 were killed at day 30 to detect the reduction of muscle larvae in diaphragms. Rats in groups C and D were killed at the same time, and all rats were bled at the same time. Specific anti-Trichinella IgG, IgG1 and IgG2a were detected by ELISA.RESULTS: Adult stage infection induced protective efficacy by 100% against adult stage and by 99.96% against larva stage. Larval stage infection induced protective efficacy by 99.92% against adult stage and 99.89% against muscle larvae. Anti-muscle stage larval ES Ag (IgG 3.0, IgG1 2.2, IgG2a 0.8) and anti-adult crude Ag antibodies (IgG 1.9, IgG1 0.8, IgG2a 0.3) significantly increased in the muscle larval stage infection compared to normal control (IgG 0.5, IgG1 0.1, IgG2a 0.1) and adult stage infection (IgG 0.5, IgG1 0.09, IgG2a 0.09) (P < 0.01). Higher specific IgG1 antibody (IgG1 2.2) in larva stage infection was shown than specific IgG2a antibody response (IgG2a 0.8)(P < 0.01).CONCLUSION: Protective immunity against both adult and larva worms has been induced from adult and muscle larva stage infections of T. spiralis.

AB - OBJECTIVE: To study the protective immunity and antibody (IgG, IgG1 and IgG2a) response against adult and larva infection of T. spiralis Korean isolate in rats.METHODS: Forty-six rats were randomly divided into 7 groups. Group A (A1, A2, 10 rats) was used for the determination of protective efficacy from adult stage infection, group B (B1, B2, 14 rats) was for the protective efficacy from muscle larva stage infection, group C (C1, C2, 17 rats) was for challenge control, and group D (5 rats) served as normal control. Rats in groups A, B and C were infected with 1,000 T.spiralis muscle larvae, and the infected rats were treated with flubendazole (20 mg/kg, 10 d) at day 7 (A1, A2) and at day 30 (B1, B2). Rats in groups A and B were re-infected with 500 T. spiralis muscle larvae at day 10 after treatment. Rats in groups A1 and B1 were killed at day 7 and day 30 to inspect the reduction of adult worms in the intestines. Rats in groups A2 and B2 were killed at day 30 to detect the reduction of muscle larvae in diaphragms. Rats in groups C and D were killed at the same time, and all rats were bled at the same time. Specific anti-Trichinella IgG, IgG1 and IgG2a were detected by ELISA.RESULTS: Adult stage infection induced protective efficacy by 100% against adult stage and by 99.96% against larva stage. Larval stage infection induced protective efficacy by 99.92% against adult stage and 99.89% against muscle larvae. Anti-muscle stage larval ES Ag (IgG 3.0, IgG1 2.2, IgG2a 0.8) and anti-adult crude Ag antibodies (IgG 1.9, IgG1 0.8, IgG2a 0.3) significantly increased in the muscle larval stage infection compared to normal control (IgG 0.5, IgG1 0.1, IgG2a 0.1) and adult stage infection (IgG 0.5, IgG1 0.09, IgG2a 0.09) (P < 0.01). Higher specific IgG1 antibody (IgG1 2.2) in larva stage infection was shown than specific IgG2a antibody response (IgG2a 0.8)(P < 0.01).CONCLUSION: Protective immunity against both adult and larva worms has been induced from adult and muscle larva stage infections of T. spiralis.

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JO - Ji sheng chong xue yu ji sheng chong bing za zhi = Journal of parasitology & parasitic diseases

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