Protective role of quercetin against cisplatin-induced hair cell damage in zebrafish embryos

S. K. Lee, K. H. Oh, A. Y. Chung, Hae Chul Park, Seung Hoon Lee, Soon Young Kwon, June Choi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background and objectives: The aim of this study was to evaluate the protective effects of quercetin on cisplatin-induced hair cell damage in transgenic zebrafish embryos. Materials and methods: Five days postfertilization zebrafish embryos were exposed to 1 mM cisplatin and quercetin at 10, 50, 100, or 200 μM for 4 h. Hair cells within neuromasts of the supraorbital, otic, and occipital lateral lines were analyzed by fluorescent microscopy (n = 10). Survival of hair cells was calculated as the average number of hair cells in the control group that were not exposed to cisplatin. Ultrastructural changes were evaluated using scanning electron microscopy. Results: Hair cell damage in neuromasts was decreased by co-treatment of quercetin and cisplatin (quercetin 100 μM: 8.6 ± 1.1 cells; 1 mM cisplatin only: 5.0 ± 0.5 cells; n = 10, p <0.05); apoptosis of hair cells examined by special stain was also decreased by quercetin. The ultrastructure of hair cells within neuromasts was preserved in zebrafish by the combination of quercetin (100 μM) and cisplatin (1 mM). Conclusion: In conclusion, quercetin showed protective effects against cisplatin-induced toxicity in a zebrafish model. The results of this study suggest the possibility of a protective role of quercetin against cisplatin-induced apoptotic cell death in zebrafish.

Original languageEnglish
Pages (from-to)1043-1052
Number of pages10
JournalHuman and Experimental Toxicology
Volume34
Issue number11
DOIs
Publication statusPublished - 2015 Nov 1

Keywords

  • cisplatin
  • hair cell
  • ototoxicity
  • Quercetin
  • zebrafish

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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