Protein biomarkers in the plasma of workers occupationally exposed to polycyclic aromatic hydrocarbons

Noh Kap-Soon, Lee Do-Youn, Jung Hak Cha, Won A. Joo, Eun Il Lee, Chan Wha Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Polycyclic aromatic hydrocarbons (PAHs) form a chemical family containing several hundred compounds, including benzo[a]pyrene and pyrene. They are usually produced by the incomplete burning of coal, oil, gas, garbage, or other organic substances like tobacco or char-broiled meat. Exposure to PAH causes tumors, primarily in the lung, the bladder, and the skin. To investigate the differentially expressed proteins resultant from PAH exposure, the protein expression in human plasma was analyzed using two-dimensional electrophoresis (2-DE). The plasma exposed to PAH was obtained from 48 waste gas pollution measurers working at an automobile emission inspection center. The 1-hydroxypryene (1-OHP) level, which is the urinary PAH metabolite used for evaluation of PAH exposure, was 0.28 μmol/mol creatinine in PAH exposure groups, and 0.078 μmol/mol creatinine in unexposed groups (control, n = 33). A protein upregulated by PAH (putative capacitative calcium entry channel) and five over-expressed proteins (two fibrinogen γ-A chain precursors, a hemopexin precursor, an albumin precursor, and T-cell receptor β chain C region) were identified with matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and confirmed with tandem MS (MS/MS) and Western blotting. The putative capacitative calcium entry channel was partially validated with a laboratory made antibody of a representative peptide fragment in PAH-exposed human plasma samples.

Original languageEnglish
Pages (from-to)3505-3513
Number of pages9
JournalProteomics
Volume4
Issue number11
DOIs
Publication statusPublished - 2004 Nov 1

Fingerprint

Polycyclic Aromatic Hydrocarbons
Biomarkers
Plasmas
Proteins
Plasma (human)
Calcium Channels
Creatinine
Gases
Hemopexin
Garbage
Calcium
Automobiles
Peptide Fragments
Tobacco
Trout
Meats
Coal
Benzo(a)pyrene
Gas oils
Metabolites

Keywords

  • 1-Hydroxypyrene
  • Benzo[a]pyrene
  • Capacitative calcium entry
  • Human plasma
  • Polycyclic aromatic hydrocarbon

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Protein biomarkers in the plasma of workers occupationally exposed to polycyclic aromatic hydrocarbons. / Kap-Soon, Noh; Do-Youn, Lee; Cha, Jung Hak; Joo, Won A.; Lee, Eun Il; Kim, Chan Wha.

In: Proteomics, Vol. 4, No. 11, 01.11.2004, p. 3505-3513.

Research output: Contribution to journalArticle

Kap-Soon, Noh ; Do-Youn, Lee ; Cha, Jung Hak ; Joo, Won A. ; Lee, Eun Il ; Kim, Chan Wha. / Protein biomarkers in the plasma of workers occupationally exposed to polycyclic aromatic hydrocarbons. In: Proteomics. 2004 ; Vol. 4, No. 11. pp. 3505-3513.
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