Protein digestibility of alkali- and fructose-treated protein by rat true digestibility assay and by the immobilized digestive enzyme assay system

Hyo-Ihl Chang, George L. Catignani, Harold E. Swaisgood

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effect of processing on the digestibility of various food proteins was examined by using the immobilized digestive enzyme assay (IDEA) system. The values obtained were compared to true digestibilities determined by rat bioassay. Sodium caseinate, egg white, soy protein, and whey were treated with either 0.2 N NaOH at 40°C for 6 h or 0.5 M fructose (pH 7.0) at 90°C for 4 h. Untreated proteins were also analyzed. Treatment of all samples (n = 12) with 4 M urea assured solubility. Regression analysis of data for all samples resulted in a correlation coefficient (r) of 0.83 (p < 0.001). The IDEA systems appears to be an accurate and reliable estimate of in vivo digestibilities. Furthermore, it offers a more rapid and less expensive alternative to animal bioassays.

Original languageEnglish
Pages (from-to)1016-1018
Number of pages3
JournalJournal of Agricultural and Food Chemistry
Volume38
Issue number4
Publication statusPublished - 1990 Dec 1
Externally publishedYes

Fingerprint

Immobilized Enzymes
Bioassay
digestive enzymes
Enzyme Assays
Alkalies
Fructose
digestible protein
alkalis
Biological Assay
Rats
fructose
Assays
digestibility
Egg Proteins
Soybean Proteins
rats
assays
Enzymes
bioassays
Caseins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Protein digestibility of alkali- and fructose-treated protein by rat true digestibility assay and by the immobilized digestive enzyme assay system. / Chang, Hyo-Ihl; Catignani, George L.; Swaisgood, Harold E.

In: Journal of Agricultural and Food Chemistry, Vol. 38, No. 4, 01.12.1990, p. 1016-1018.

Research output: Contribution to journalArticle

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