Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm

Jong Heon Kim, Bumsuk Hahm, Yoon Ki Kim, Mieyoung Choi, Sung Key Jang

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are involved in several RNA-related biological processes such as transcription, pre-mRNA processing, mature mRNA transport to the cytoplasm, and translation. About 20 major hnRNPs from A1 to U are known. Among them, hnRNP A, D, E, I, and K are known to shuttle between the nucleus and the cytoplasm. hnRNP E2 has been seen to stabilize α-globin mRNA and to enhance polioviral mRNA translation. hnRNP K modulates transcription and translation of some mRNAs, hnRNP I and its homologue hnRNP L have been suggested to enhance translation of some IRES-dependent mRNAs. In order to better understand the molecular mechanisms of the biological functions of hnRNPs, we investigated protein-protein interactions of six hnRNPs (hnRNP A1, C1, E2, I, K, and L) using the yeast two-hybrid system and in vitro co-precipitation assays. All of the hnRNPs tested exerted homomeric interactions, and hnRNP E2, I, K, and L interacted with each other. In the case of hnRNP E2 and hnRNP K, the N-terminal half of the proteins containing two KH (K homologous) domains were required for protein-protein interaction, and the second quarter of hnRNP I and hnRNP L containing RRM2 (RNA recognition motif 2) was essential for protein-protein interaction, hnRNP A1 and C1 did not form complexes with other hnRNPs in our assay systems. This suggests that the hnRNPs could fall into two groups: one group, including hnRNP A1 and C1, involved in hnRNP core complex formation and another group, including hnRNP E2, I, K, and L, involved in a variety of RNA-related biological processes. Different combinations of the proteins of the second group may facilitate different biological processes in conjunction with other factors. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)395-405
Number of pages11
JournalJournal of Molecular Biology
Volume298
Issue number3
DOIs
Publication statusPublished - 2000 May 5
Externally publishedYes

Fingerprint

Heterogeneous-Nuclear Ribonucleoproteins
Cytoplasm
Proteins
Biological Phenomena
Heterogeneous-Nuclear Ribonucleoprotein L
Heterogeneous-Nuclear Ribonucleoprotein K
Protein Biosynthesis
Messenger RNA
RNA
Two-Hybrid System Techniques
Globins
RNA Precursors

Keywords

  • In vitro co-precipitation
  • Protein-protein interaction
  • RNA-related processes
  • Shuttling hnRNPs
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Virology

Cite this

Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm. / Kim, Jong Heon; Hahm, Bumsuk; Kim, Yoon Ki; Choi, Mieyoung; Jang, Sung Key.

In: Journal of Molecular Biology, Vol. 298, No. 3, 05.05.2000, p. 395-405.

Research output: Contribution to journalArticle

Kim, Jong Heon ; Hahm, Bumsuk ; Kim, Yoon Ki ; Choi, Mieyoung ; Jang, Sung Key. / Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm. In: Journal of Molecular Biology. 2000 ; Vol. 298, No. 3. pp. 395-405.
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