Proteome analysis of adipocyte lipid rafts reveals that gClqR plays essential roles in adipogenesis and insulin signal transduction

Ki Bum Kim, Bong Woo Kim, Hyo Jung Choo, Young Chan Kwon, Byung Yoon Ahn, Jong Soon Choi, Jae Seon Lee, Young Gyu Ko

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Since insulin receptors and their downstream signaling molecules are organized in lipid rafts, proteomic analysis of adipocyte lipid rafts may provide new insights into the function of lipid rafts in adipogenesis and insulin signaling. To search for proteins involved in adipocyte differentiation and insulin signaling, we analyzed detergent-resistant lipid raft proteins from 3T3-L1 preadipocytes and adipocytes by 2-DE. Eleven raft proteins were identified from adipocytes. One of the adipocyte-specific proteins was globular C1q receptor (gC1qR), an acidic 32 kDa protein known as the receptor for the globular domain of complement C1q. The targeting of gC1qR into lipid rafts was significantly increased during adipogenesis, as determined by immunoblotting and immunofluorescence. Since the silencing of gC1qR by small RNA interference abolished adipogenesis and blocked insulin-induced activation of insulin receptor, insulin receptor sub-strate-1 (IRS-1), Akt, and Erk1/2, we can conclude that gC1qR is an essential molecule involved in adipogenesis and insulin signaling.

Original languageEnglish
Pages (from-to)2373-2382
Number of pages10
JournalProteomics
Volume9
Issue number9
DOIs
Publication statusPublished - 2009 May

Keywords

  • Insulin resistance
  • Lipid raft
  • Signal transduction
  • Two-dimensional difference gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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