Proteome analysis of Halobacterium salinarum and characterization of proteins related to the degradation of isopropyl alcohol

Dong J. Ha, Won A. Joo, Gi Yeon Han, Chan Wha Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We reported in a previous study that proteomic approach, coupled with genomic techniques, could be used to screen and develop multiple candidates for halophilic enzymes from Halobacterium salinarum. In order to evaluate the biodegradation of isopropyl alcohol (IPA) by H. salinarum, the amounts of residual IPA and acetone generated in the growth media were determined using a gas chromatography-flame ionization detector (GC-FID). The protein expression profiles of cells which had been cultured with IPA were obtained with the two-dimensional gel electrophoresis. Proteins evidencing different expression levels in the presence of 0.5% IPA were identified by electrospray ionization-quadruple-time of flight (ESI-Q-TOF) mass spectrometry. We found 12 proteins which were down-regulated, and another 12 proteins which were up-regulated, in the presence of 0.5% IPA and we further identified 17 proteins among them using ESI-TOF MS/MS. Among these identified proteins, we selected glyceraldehyde 3-phosphate dehydrogenase (GAPDH) for further characterization as a halophilic enzyme. We have demonstrated for the first time that H. salinarum possesses the ability to degrade IPA and GAPDH was both stable and active at high salt concentrations, with maximum activity occurring at 1 M NaCl, although the optimal salt concentration with regard to the growth of H. salinarum is 4.3 M.

Original languageEnglish
Pages (from-to)44-50
Number of pages7
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1774
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

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Halobacterium salinarum
2-Propanol
Proteome
Degradation
Glyceraldehyde-3-Phosphate Dehydrogenases
Proteins
Salts
Flame Ionization
Electrospray ionization
Electrophoresis, Gel, Two-Dimensional
Enzymes
Growth
Acetone
Biodegradation
Electrophoresis
Gas chromatography
Gas Chromatography
Proteomics
Ionization
Mass spectrometry

Keywords

  • 2-DE
  • ESI-Q-TOF
  • GAPDH
  • GC-FID
  • Halobacterium salinarum

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics

Cite this

Proteome analysis of Halobacterium salinarum and characterization of proteins related to the degradation of isopropyl alcohol. / Ha, Dong J.; Joo, Won A.; Han, Gi Yeon; Kim, Chan Wha.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1774, No. 1, 01.01.2007, p. 44-50.

Research output: Contribution to journalArticle

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