Proteome analysis of serum from type 2 diabetics with nephropathy

Hyun Jung Kim, Eun Hee Cho, Ji Hye Yoo, Pan Kyeom Kim, Jun Seop Shin, Mi Ryung Kim, Chan Wha Kim

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Diabetic nephropathy (DN) is a renal disease which develops as a consequence of diabetes mellitus. Microalbuminuria is the earliest clinical sign of DN. There are no specific diagnostic biomarkers for type 2 diabetics with nephropathy other than microalbuminuria and macroalbuminuria. However, microalbuminuria does not constitute a sole independent indicator for type 2 diabetics with nephropathy, and thus, another screening method, such as a biomarker assay, is required in order to diagnose it more correctly. Therefore, we have utilized two-dimensional electrophoresis (2-DE) to identify human serum protein markers for the more specific and accurate prediction of progressive nephropathy in type 2 diabetes patients, via comparisons of the serum proteome in three experimental groups: type 2 diabetes patients without microalbuminuria (DM, n = 30), with microalbuminuria (MA, n = 29), and with chronic renal failure (CRF, n = 31). As a result, proteins which were differentially expressed with statistical significance (p < 0.05) in MA and CRF groups as compared to those in DM group were selected and identified by ESI-Q-TOF MS/MS. Among these identified proteins, two proteins which might be useful as diagnostic biomarkers of type 2 diabetics with nephropathy were verified by Western blotting: extracellular glutathione peroxidase (eGPx) and apolipoprotein (ApoE) were found to exhibit a progressive reduction in MA and CRF groups. Notably, eGPx was further verified by ELISA using DM (n = 100) and MA (n = 96) patient samples. Collectively, our results show that the two proteins identified in this study may constitute potential biomarkers for the diagnosis of type 2 diabetics with nephropathy.

Original languageEnglish
Pages (from-to)735-743
Number of pages9
JournalJournal of Proteome Research
Volume6
Issue number2
DOIs
Publication statusPublished - 2007 Feb

Keywords

  • 2-DE
  • ApoE
  • Diabetic nephropathy
  • ESI-Q-TOF MS/MS
  • Serum
  • eGPx

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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  • Cite this

    Kim, H. J., Cho, E. H., Yoo, J. H., Kim, P. K., Shin, J. S., Kim, M. R., & Kim, C. W. (2007). Proteome analysis of serum from type 2 diabetics with nephropathy. Journal of Proteome Research, 6(2), 735-743. https://doi.org/10.1021/pr060489g