Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose

Pan Kyeom Kim, Mi Ryung Kim, Hyun Jung Kim, Hyun Syuk Yoo, Ji Soo Kim, Eun Hee Cho, Chan Wha Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2-DE and ESI-Q-TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.

Original languageEnglish
Pages (from-to)2184-2188
Number of pages5
JournalProteomics
Volume7
Issue number13
DOIs
Publication statusPublished - 2007 Jun 1

Keywords

  • Electrospray-quadrupole-time-of-flight mass spectrometry
  • Hepatic stellate cell
  • High glucose
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Kim, P. K., Kim, M. R., Kim, H. J., Yoo, H. S., Kim, J. S., Cho, E. H., & Kim, C. W. (2007). Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose. Proteomics, 7(13), 2184-2188. https://doi.org/10.1002/pmic.200700051