Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose

Pan Kyeom Kim; Mi Ryung Kim; Hyun Jung Kim; Hyun Syuk Yoo; Ji Soo Kim; Eun Hee Cho; Chan Wha Kim

doi:10.1002/pmic.200700051

Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose

Pan Kyeom Kim, Mi Ryung Kim, Hyun Jung Kim, Hyun Syuk Yoo, Ji Soo Kim, Eun Hee Cho, Chan Wha Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2-DE and ESI-Q-TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.

Original language	English
Pages (from-to)	2184-2188
Number of pages	5
Journal	Proteomics
Volume	7
Issue number	13
DOIs	https://doi.org/10.1002/pmic.200700051
Publication status	Published - 2007 Jun

Keywords

Electrospray-quadrupole-time-of-flight mass spectrometry
Hepatic stellate cell
High glucose
Two-dimensional gel electrophoresis

ASJC Scopus subject areas

Biochemistry
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/pmic.200700051

Cite this

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abstract = "To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2-DE and ESI-Q-TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.",

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author = "Kim, {Pan Kyeom} and Kim, {Mi Ryung} and Kim, {Hyun Jung} and Yoo, {Hyun Syuk} and Kim, {Ji Soo} and Cho, {Eun Hee} and Kim, {Chan Wha}",

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AU - Kim, Pan Kyeom

AU - Kim, Mi Ryung

AU - Kim, Hyun Jung

AU - Yoo, Hyun Syuk

AU - Kim, Ji Soo

AU - Cho, Eun Hee

AU - Kim, Chan Wha

PY - 2007/6

Y1 - 2007/6

N2 - To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2-DE and ESI-Q-TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.

AB - To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2-DE and ESI-Q-TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.

KW - Electrospray-quadrupole-time-of-flight mass spectrometry

KW - Hepatic stellate cell

KW - High glucose

KW - Two-dimensional gel electrophoresis

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ER -

Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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