To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2-DE and ESI-Q-TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.
- Electrospray-quadrupole-time-of-flight mass spectrometry
- Hepatic stellate cell
- High glucose
- Two-dimensional gel electrophoresis
ASJC Scopus subject areas
- Molecular Biology