Proteome analysis of virulence factor regulated by autoinducer-2-like activity in Escherichia coli O157

H7

Younghoon Kim, Sangnam Oh, Eun Young Ahn, Jee Young Imm, Sejong Oh, Sungsu Park, Sae Hun Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Many pathogenic bacteria, including Escherichia coli O157:H7, can control gene expression in a cell density-dependent manner by producing small signaling molecules (autoinducers) in a process known as quorum sensing. In this study, the effects of the autoinducer-2-like activity on the expression of proteins, including virulence factors, in E. coli O157:H7 were characterized by proteomic analysis. Compared with the control, E. coli O157:H7 strains in the presence of autoinducer-2-like activity exhibited elevated virulence by more rapidly forming cell aggregates on epithelial cells and rapidly killing the nematode Caenorhabditis elegans, the surrogate host. Two-dimensional gel electrophoresis revealed 18 proteins that were upregulated by autoinducer-2-like activity and 4 proteins that were down-regulated. These proteins were further characterized by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry and are involved in the metabolic process, adaptation and protection, cell motility, secretion, envelope biogenesis, and protein translation. These results indicate that the newly identified proteins are associated with the control of virulence in E. coli O157:H7 and that these proteins can be potential targets for the development of antibiotics and other antimicrobial agents.

Original languageEnglish
Pages (from-to)300-307
Number of pages8
JournalJournal of Food Protection
Volume70
Issue number2
Publication statusPublished - 2007 Feb 1

Fingerprint

Escherichia coli O157
Virulence Factors
Proteome
proteome
virulence
Proteins
proteins
Virulence
Quorum Sensing
quorum sensing
matrix-assisted laser desorption-ionization mass spectrometry
two-dimensional gel electrophoresis
Electrophoresis, Gel, Two-Dimensional
Caenorhabditis elegans
Protein Biosynthesis
cell aggregates
Anti-Infective Agents
cell movement
Proteomics
proteomics

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Biotechnology

Cite this

Proteome analysis of virulence factor regulated by autoinducer-2-like activity in Escherichia coli O157 : H7. / Kim, Younghoon; Oh, Sangnam; Ahn, Eun Young; Imm, Jee Young; Oh, Sejong; Park, Sungsu; Kim, Sae Hun.

In: Journal of Food Protection, Vol. 70, No. 2, 01.02.2007, p. 300-307.

Research output: Contribution to journalArticle

Kim, Younghoon ; Oh, Sangnam ; Ahn, Eun Young ; Imm, Jee Young ; Oh, Sejong ; Park, Sungsu ; Kim, Sae Hun. / Proteome analysis of virulence factor regulated by autoinducer-2-like activity in Escherichia coli O157 : H7. In: Journal of Food Protection. 2007 ; Vol. 70, No. 2. pp. 300-307.
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