Proteome response of Escherichia coli fed-batch culture to temperature downshift

Yang Hoon Kim, Kyung Yeon Han, Kibeom Lee, Jeewon Lee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

During fed-batch cultivation of Escherichia coli K-12, the proteomic response to a temperature downshift from 37 to 20°C was quantitatively monitored and analyzed by using two-dimensional electrophoresis. When the temperature of exponentially growing E. coli K-12 culture was downshifted to 20°C, the synthesis level of 57 intracellular proteins showed significant changes for a prolonged period of time, compared to the fed-batch culture controlled at 37°C. Thus, these proteins are regarded as important stress proteins responsive to cold shock, which were analyzed by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and identified using the E. coli SWISS-2DPAGE database. Most of the identified proteins were shown to be involved in energy metabolism, several cellular molecule biosynthetic pathways and catabolism, cell processes, flagellar biosynthesis and motility, and protein translation and folding. The systematic approach to the monitoring of proteomic responses and the detailed analysis results reported in this article would be useful in understanding the metabolic adaptation to lowered culture temperature and designing efficient fermentation strategies for the production of recombinant proteins and metabolites using E. coli strains.

Original languageEnglish
Pages (from-to)786-793
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume68
Issue number6
DOIs
Publication statusPublished - 2005 Oct 1

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ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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